Panorganismal Metabolic Response Modeling of an Experimental Echinostoma caproni Infection in the Mouse
文献类型：期刊论文
| 作者 | Saric, Jasmina1,2; Li, Jia V.1,2; Wang, Yulan2,3; Keiser, Jennifer4; Veselkov, Kirill2; Dirnhofer, Stephan5; Yap, Ivan K. S.2; Nicholson, Jeremy K.2; Holmes, Elaine2; Utzinger, Juerg1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2009-08-01 |
| 卷号 | 8期号:8页码:3899-3911 |
| 关键词 | Biomarker Cluster Analysis Echinostoma caproni Hierarchical Analysis Magic Angle Spinning Metabolic Profiling NMR Spectroscopy Systemic Effect |
| 通讯作者 | Utzinger, J (reprint author), Swiss Trop Inst, Dept Publ Hlth & Epidemiol, CH-4002 Basel, Switzerland. |
| 英文摘要 | Metabolic profiling of host tissues and biofluids during parasitic infections can reveal new biomarker information and aid the elucidation of mechanisms of disease. The multicompartmental metabolic effects of an experimental Echinostoma caproni infection have been characterized in 12 outbred female mice infected orally with 30 E caproni metacercariae each, using a further 12 uninfected animals as a control group. Mice were killed 36 days postinfection and brain, intestine (colon, ileum, jejeunum), kidney, liver, and spleen were removed. Metabolic profiles of tissue samples were measured using high-resolution magic angle spinning (1)H NMR spectroscopy and biofluids measured by applying conventional (1)H NMR spectroscopy. Spectral data were analyzed via principal component analysis, partial least-squares-derived methods and hierarchical projection analyses. Infection-induced metabolic changes in the tissues were correlated with altered metabolite concentrations in the biofluids (urine, plasma, fecal water) using hierarchical modeling and correlation analyses. Metabolic descriptors of infection were identified in liver, renal cortex, intestinal tissues but not in spleen, brain or renal medulla. The main physiological change observed in the mouse was malabsorption in the small intestine, which was evidenced by decreased levels of various amino acids in the ileum, for example, alanine, taurine, glutamine, and branched chain amino acids. Furthermore, altered gut microbial activity or composition was reflected by increased levels of trimethylamine in the colon. Our modeling approach facilitated in-depth appraisal of the covariation of the metabolic profiles of different biological matrices and found that urine and plasma most closely reflected changes in ileal compartments. In conclusion, an E. caproni infection not only results in direct localized (ileum and jejenum) effects, but also causes remote metabolic changes (colon and several peripheral organs), and therefore describes the panorganismal metabolic response of the infection. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; SPINNING H-1-NMR SPECTROSCOPY ; HIGH-RESOLUTION ; GASTROINTESTINAL PARASITISM ; BIOMARKER IDENTIFICATION ; SCHISTOSOMA-MANSONI ; NMR-SPECTROSCOPY ; AMINO-ACIDS ; DATA SETS ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268661800013 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2189]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Swiss Trop Inst, Dept Publ Hlth & Epidemiol, CH-4002 Basel, Switzerland 2.Univ London Imperial Coll Sci Technol & Med, Dept Biomol Med, Div Surg Oncol Reprod Biol & Anaesthet SORA, Fac Med, London SW7 2AZ, England 3.Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 4.Swiss Trop Inst, Dept Med Parasitol & Infect Biol, CH-4002 Basel, Switzerland 5.Univ Basel Hosp, Inst Pathol, CH-4031 Basel, Switzerland |
| 推荐引用方式 GB/T 7714 | Saric, Jasmina,Li, Jia V.,Wang, Yulan,et al. Panorganismal Metabolic Response Modeling of an Experimental Echinostoma caproni Infection in the Mouse[J]. JOURNAL OF PROTEOME RESEARCH,2009,8(8):3899-3911. |
| APA | Saric, Jasmina.,Li, Jia V..,Wang, Yulan.,Keiser, Jennifer.,Veselkov, Kirill.,...&Utzinger, Juerg.(2009).Panorganismal Metabolic Response Modeling of an Experimental Echinostoma caproni Infection in the Mouse.JOURNAL OF PROTEOME RESEARCH,8(8),3899-3911. |
| MLA | Saric, Jasmina,et al."Panorganismal Metabolic Response Modeling of an Experimental Echinostoma caproni Infection in the Mouse".JOURNAL OF PROTEOME RESEARCH 8.8(2009):3899-3911. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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