Saturation transfer difference nuclear magnetic resonance study on the specific binding of ligand to protein
文献类型：期刊论文
| 作者 | Ji, Zhusheng1; Yao, Zhongxiu1,2; Liu, Maili1 |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2009-02-15 |
| 卷号 | 385期号:2页码:380-382 |
| 通讯作者 | Ji, ZS (reprint author), Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China. |
| 英文摘要 | Ligand-based nuclear magnetic resonance (NMR) approaches have shown great promise in the study of ligand-protein interaction. But these approaches suffer from interference from the nonspecific binding. Here a saturation transfer difference (STD) NMR method to map the group epitope and to measure the dissociation constant (K(D)) of specific interaction between ligand and protein is presented. The interference from nonspecific binding was corrected by recording STD NMR spectra of ligand-protein solutions with and without inhibitor saturating the mutually specific binding site and subtracting one from the other. The method was examined With L-tryptophan (Trp), naproxen (Nap), and human serum albumin (HSA) as model ligand, inhibitor, and protein, respectively. Results agree well with other reports of Trp-HSA interaction. (C) 2008 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | HIGH-AFFINITY LIGANDS ; NMR-SPECTROSCOPY ; SERUM-ALBUMIN ; RECEPTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262877400031 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/2269]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Zhusheng,Yao, Zhongxiu,Liu, Maili. Saturation transfer difference nuclear magnetic resonance study on the specific binding of ligand to protein[J]. ANALYTICAL BIOCHEMISTRY,2009,385(2):380-382. |
| APA | Ji, Zhusheng,Yao, Zhongxiu,&Liu, Maili.(2009).Saturation transfer difference nuclear magnetic resonance study on the specific binding of ligand to protein.ANALYTICAL BIOCHEMISTRY,385(2),380-382. |
| MLA | Ji, Zhusheng,et al."Saturation transfer difference nuclear magnetic resonance study on the specific binding of ligand to protein".ANALYTICAL BIOCHEMISTRY 385.2(2009):380-382. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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