F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells
文献类型：期刊论文
| 作者 | Ye, Yansheng1,2; Liu, Xiaoli1; Zhang, Zeting1; Wu, Qiong1; Jiang, Bin1 ; Jiang, Ling1; Zhang, Xu1; Liu, Maili1; Pielak, Gary J.3; Li, Conggang1
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| 刊名 | CHEMISTRY-A EUROPEAN JOURNAL
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| 出版日期 | 2013-09-16 |
| 卷号 | 19期号:38页码:12705-12710 |
| 关键词 | cytoplasmic viscosity fluorine NMR spectroscopy transient weak interactions |
| 英文摘要 | Protein mobility in living cells is vital for cell function. Both cytosolic viscosity and weak protein-protein interactions affect mobility, but examining viscosity and weak interaction effects is challenging. Herein, we demonstrate the use of (FNMR)-F-19 spectroscopy to measure cytoplasmic viscosity and to characterize nonspecific protein-protein interactions in living Escherichiacoli cells. The origins of resonance broadening in Escherichiacoli cells were also investigated. We found that sample inhomogeneity has a negligible effect on resonance broadening, the cytoplasmic viscosity is only about 2-3 times that of water, and ubiquitous transient weak protein-protein interactions in the cytosol play a significant role in governing the detection of proteins by using in-cell NMR spectroscopy. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; FLUOROTYROSINE ALKALINE-PHOSPHATASE ; MODEL-FREE APPROACH ; ESCHERICHIA-COLI ; IN-VIVO ; ROTATIONAL MOBILITY ; RELAXATION ; DIFFUSION ; CONSEQUENCES ; MACROMOLECULES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324316300023 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/808]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100029, Peoples R China 3.Univ N Carolina, Dept Biochem & Biophys, Dept Chem, Chapel Hill, NC 27599 USA |
| 推荐引用方式 GB/T 7714 | Ye, Yansheng,Liu, Xiaoli,Zhang, Zeting,et al. F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells[J]. CHEMISTRY-A EUROPEAN JOURNAL,2013,19(38):12705-12710. |
| APA | Ye, Yansheng.,Liu, Xiaoli.,Zhang, Zeting.,Wu, Qiong.,Jiang, Bin.,...&Li, Conggang.(2013).F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.CHEMISTRY-A EUROPEAN JOURNAL,19(38),12705-12710. |
| MLA | Ye, Yansheng,et al."F-19 NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells".CHEMISTRY-A EUROPEAN JOURNAL 19.38(2013):12705-12710. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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