NMR Spectroscopic Approach Reveals Metabolic Diversity of Human Blood Plasma Associated with Protein-Drug Interaction
文献类型：期刊论文
| 作者 | Du, Yuanyuan1; Lan, Wenxian2; Ji, Zhusheng1; Zhang, Xu1; Jiang, Bin1 ; Zhou, Xin1; Li, Conggang1; Liu, Maili1
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2013-09-17 |
| 卷号 | 85期号:18页码:8601-8608 |
| 英文摘要 | Although blood plasma has been used to diagnose diseases and to evaluate physiological conditions, it is not easy to establish a global normal concentration range for the targeting components in the plasma due to the inherent metabolic diversity. We show here that NMR spectroscopy coupled with principal component analysis (PCA) may provide a useful method for quantitatively characterizing the metabolic diversity of human blood plasma. We analyzed 70 human blood plasma samples with and without addition of ibuprofen. By defining the PC score values as diversity index (I-div) and the drug-induced PC score value change as interaction index (I-dist), we find that the two indexes are highly correlated (P < 0.0001). Triglycerides, choline-containing phospholipids, lactate, and pyruvate are associated with both indexes (P < 0.0001), respectively. In addition, a significant amount of lactate and pyruvate are in the NMR "invisible" bound forms and can be replaced by ibuprofen. The diffusion and transverse relaxation time weighted NMR approaches gave rise to a better characterization of the diversity and the interaction than that of the one acquired using NOESYPR1D with 100 ms mixing time. These results might be useful for understanding the blood plasma drug interaction and personalized therapy. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; HUMAN SERUM-ALBUMIN ; PATTERN-RECOGNITION METHODS ; LOW-AFFINITY BINDING ; H-1-NMR SPECTROSCOPY ; DIFFUSION-COEFFICIENTS ; RELAXATION ; IBUPROFEN ; IDENTIFICATION ; LIPOPROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330017400019 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/840]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Yuanyuan,Lan, Wenxian,Ji, Zhusheng,et al. NMR Spectroscopic Approach Reveals Metabolic Diversity of Human Blood Plasma Associated with Protein-Drug Interaction[J]. ANALYTICAL CHEMISTRY,2013,85(18):8601-8608. |
| APA | Du, Yuanyuan.,Lan, Wenxian.,Ji, Zhusheng.,Zhang, Xu.,Jiang, Bin.,...&Liu, Maili.(2013).NMR Spectroscopic Approach Reveals Metabolic Diversity of Human Blood Plasma Associated with Protein-Drug Interaction.ANALYTICAL CHEMISTRY,85(18),8601-8608. |
| MLA | Du, Yuanyuan,et al."NMR Spectroscopic Approach Reveals Metabolic Diversity of Human Blood Plasma Associated with Protein-Drug Interaction".ANALYTICAL CHEMISTRY 85.18(2013):8601-8608. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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