Biomolecular ligands screening using radiation damping difference WaterLOGSY spectroscopy
文献类型：期刊论文
| 作者 | Sun, Peng1,2; Jiang, Xianwang1,2; Jiang, Bin1 ; Zhang, Xu1; Liu, Maili1
|
| 刊名 | JOURNAL OF BIOMOLECULAR NMR
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 56期号:3页码:285-290 |
| 关键词 | NMR Radiation damping Ligand screening WaterLOGSY |
| 英文摘要 | Water-ligand observed via gradient spectroscopy (WaterLOGSY) is a widely used nuclear magnetic resonance method for ligand screening. The crucial procedure for the effectiveness of WaterLOGSY is selective excitation of the water resonance. The selective excitation is conventionally achieved by using long selective pulse, which causes partial saturation of the water magnetization leading to reduction of sensitivity, in addition to time consuming and error prone. Therefore, many improvements have been made to enhance the sensitivity and robustness of the method. Here we propose an alternative selective excitation scheme for WaterLOGSY by utilizing radiation damping effect. The pulse scheme starts simply with a hard inversion pulse, instead of selective pulse or pulse train, followed by a pulse field gradient to control the radiation damping effect. The rest parts of the pulse scheme are similar to conventional WaterLOGSY. When the gradient pulse is applied immediately after the inversion pulse, the radiation damping effect is suppressed, and all of the magnetization is inversed. When the gradient pulse and the inversion pulse are about 10-20 ms apart, the radiation damping effect remains active and drives the water magnetization toward +z-axis, resulting in selective non-inversion of the water magnetization. By taking the differences of the spectra obtained under these two conditions, one should get the result of WaterLOGSY. The method is demonstrated to be simple, robust and sensitive for ligand screening. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Spectroscopy |
| 关键词[WOS] | BINDING-AFFINITY ; PROTEIN-RECEPTOR ; NMR-SPECTROSCOPY ; WATERGATE PULSE ; DRUG DISCOVERY ; EXCHANGE RATES ; FLIP-BACK ; SEQUENCE ; MACROMOLECULES ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321544600009 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/963]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Peng,Jiang, Xianwang,Jiang, Bin,et al. Biomolecular ligands screening using radiation damping difference WaterLOGSY spectroscopy[J]. JOURNAL OF BIOMOLECULAR NMR,2013,56(3):285-290. |
| APA | Sun, Peng,Jiang, Xianwang,Jiang, Bin,Zhang, Xu,&Liu, Maili.(2013).Biomolecular ligands screening using radiation damping difference WaterLOGSY spectroscopy.JOURNAL OF BIOMOLECULAR NMR,56(3),285-290. |
| MLA | Sun, Peng,et al."Biomolecular ligands screening using radiation damping difference WaterLOGSY spectroscopy".JOURNAL OF BIOMOLECULAR NMR 56.3(2013):285-290. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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