Direct visualization of the dynamics of membrane-anchor proteins in living cells
文献类型：期刊论文
| 作者 | Wang C. ; Fu G. ; Wang J. ; Wang G. ; Cheng Y. ; Xu Z. Z. |
| 刊名 | j. microsc.-oxf.
 |
| 出版日期 | 2008 |
| 卷号 | 229期号:1页码:67 |
| 关键词 | green fluorescence protein living cells oblique illumination single-molecule single-particle total internal reflection illumination tracking |
| ISSN号 | 0022-2720 |
| 中文摘要 | dynamic properties of proteins have crucial roles in understanding protein function and molecular mechanism within cells. in this paper, we combined total internal reflection fluorescence microscopy with oblique illumination fluorescence microscopy to observe directly the movement and localization of membrane-anchored green fluorescence proteins in living cells. total internal reflect illumination allowed the observation of proteins in the cell membrane of living cells since the penetrate depth could be adjusted to about 80 nm, and oblique illumination allowed the observation of proteins both in the cytoplasm and apical membrane, which made this combination a promising tool to investigate the dynamics of proteins through the whole cell. not only individual protein molecule tracks have been analyzed quantitatively but also cumulative probability distribution function analysis of ensemble trajectories has been done to reveal the mobility of proteins. finally, single particle tracking has acted as a compensation for single molecule tracking. all the results exhibited green fluorescence protein dynamics within cytoplasm, on the membrane and from cytoplasm to plasma membrane. |
| 学科主题 | 激光技术;激光物理与基本理论 |
| 语种 | 英语 |
| WOS记录号 | WOS:000251739600010 |
| 公开日期 | 2009-09-18 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/504]  |
| 专题 | 上海光学精密机械研究所_强场激光物理国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang C.,Fu G.,Wang J.,et al. Direct visualization of the dynamics of membrane-anchor proteins in living cells[J]. j. microsc.-oxf.,2008,229(1):67, 77. |
| APA | Wang C.,Fu G.,Wang J.,Wang G.,Cheng Y.,&Xu Z. Z..(2008).Direct visualization of the dynamics of membrane-anchor proteins in living cells.j. microsc.-oxf.,229(1),67. |
| MLA | Wang C.,et al."Direct visualization of the dynamics of membrane-anchor proteins in living cells".j. microsc.-oxf. 229.1(2008):67. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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