Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research
文献类型：期刊论文
| 作者 | Wang, Guifang1; Zhang, Ze-Ting2,3; Jiang, Bin3 ; Zhang, Xu3; Li, Conggang2,3; Liu, Maili3
|
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 406期号:9-10页码:2279-2288 |
| 关键词 | Protein NMR Protein therapeutics Posttranslational modifications Protein aggregation |
| 英文摘要 | Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography are the two main methods for protein three-dimensional structure determination at atomic resolution. According to the protein structures deposited in the Protein Data Bank, X-ray crystallography has become the dominant method for structure determination, particularly for large proteins and complexes. However, with the developments of isotope labeling, increase of magnetic field strength, common use of a cryogenic probe, and ingenious pulse sequence design, the applications of NMR spectroscopy have expanded in biological research, especially in characterizing protein dynamics, sparsely populated transient structures, weak protein interactions, and proteins in living cells at atomic resolution, which is difficult if not impossible by other biophysical methods. Although great advances have been made in protein NMR spectroscopy, its applications in protein therapeutics, which represents the fastest growing segment of the pharmaceutical industry, are still limited. Here we review the recent advances in the use of NMR spectroscopy in studies of large proteins or complexes, posttranslation modifications, weak interactions, and aggregation, and in-cell NMR spectroscopy. The potential applications of NMR spectroscopy in protein therapeutic assays are discussed. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; HIGH-RESOLUTION NMR ; PARAMAGNETIC RELAXATION ENHANCEMENT ; IN-CELL ; LIVING CELLS ; ESCHERICHIA-COLI ; STATE NMR ; F-19 NMR ; DYNAMICS ; BIOPHARMACEUTICALS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334171200005 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1321]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Branch, Natl Sci Lib, Wuhan Documentat & Informat Ctr, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Key Lab Magnet Resonance Biol Syst, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Guifang,Zhang, Ze-Ting,Jiang, Bin,et al. Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2014,406(9-10):2279-2288. |
| APA | Wang, Guifang,Zhang, Ze-Ting,Jiang, Bin,Zhang, Xu,Li, Conggang,&Liu, Maili.(2014).Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,406(9-10),2279-2288. |
| MLA | Wang, Guifang,et al."Recent advances in protein NMR spectroscopy and their implications in protein therapeutics research".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406.9-10(2014):2279-2288. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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