Interferon regulatory factor 8 protects against cerebral ischaemic-reperfusion injury
文献类型：期刊论文
| 作者 | Xiang, Mei1,2; Wang, Lang1,2; Guo, Sen1,2; Lu, Yan-Yun1,2; Lei, Hao3; Jiang, Ding-Sheng1,2; Zhang, Yan1,2; Liu, Yi4; Zhou, Yan4; Zhang, Xiao-Dong4 |
| 刊名 | JOURNAL OF NEUROCHEMISTRY
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 129期号:6页码:988-1001 |
| 关键词 | apoptosis inflammation IRF8 ischaemia oxidative stress stroke |
| 英文摘要 | Interferon regulatory factor 8 (IRF8), a transcriptional regulator in the IRF family, has been implicated in innate immunity, immune cell differentiation and tumour cell apoptosis. In the present study, we found that IRF8 is constitutively expressed in the brain and suppressed after cerebral ischaemia in a time-dependent manner. IRF8 knockout (IRF8-KO) mice, wild type (WT) mice, neuron-specific IRF8 transgenic (TG) mice and non-transgenic mice were used in a transient cerebral ischaemic model. The IRF8 knockout mice exhibited aggravated apoptosis, inflammation and oxidative injury in the ischaemic brain, eventually leading to poorer stroke outcomes, whereas neuron-specific IRF8 transgenic mice showed a marked inhibition of apoptosis and improved stroke outcomes. To model ischaemia/reperfusion conditions in vitro, primary cortical neurons were cultured and subjected to transient oxygen and glucose deprivation for 60min. Similar to the in vivo study, IRF8 knockdown by Ad-shIRF8 resulted in increased apoptosis, whereas IRF8 over-expression by Ad-IRF8 significantly decreased neuronal apoptosis. These data indicate that IRF8 is strongly protective in ischaemic stroke by regulating neuronal apoptosis, the inflammatory response and oxidative stress. In the present study, we found that the transcriptional factor IRF8 plays a protective role in the cerebral ischaemic-reperfusion injury by attenuating neuronal apoptosis, oxidative stress and inflammation. Besides the known function of IRF8 in regulating the inflammatory gene expression, we first demonstrated that IRF8 can directly modulate apoptosis and oxidative stress by controlling the relative genes expression. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Neurosciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
| 关键词[WOS] | SEQUENCE BINDING-PROTEIN ; PATHOLOGICAL CARDIAC-HYPERTROPHY ; CRITICAL TRANSCRIPTION FACTOR ; BRAIN-INJURY ; IRF FAMILY ; EXPERIMENTAL STROKE ; MULTIPLE-SCLEROSIS ; NEGATIVE REGULATOR ; OXIDATIVE STRESS ; TUMOR-CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337760500009 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1382]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Wuhan Univ, Renmin Hosp, Dept Cardiol, Wuhan 430060, Peoples R China 2.Wuhan Univ, Cardiovasc Res Inst, Wuhan 430060, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance, Wuhan, Peoples R China 4.Wuhan Univ, Coll Life Sci, Wuhan 430060, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Mei,Wang, Lang,Guo, Sen,et al. Interferon regulatory factor 8 protects against cerebral ischaemic-reperfusion injury[J]. JOURNAL OF NEUROCHEMISTRY,2014,129(6):988-1001. |
| APA | Xiang, Mei.,Wang, Lang.,Guo, Sen.,Lu, Yan-Yun.,Lei, Hao.,...&Li, Hongliang.(2014).Interferon regulatory factor 8 protects against cerebral ischaemic-reperfusion injury.JOURNAL OF NEUROCHEMISTRY,129(6),988-1001. |
| MLA | Xiang, Mei,et al."Interferon regulatory factor 8 protects against cerebral ischaemic-reperfusion injury".JOURNAL OF NEUROCHEMISTRY 129.6(2014):988-1001. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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