The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B
文献类型：期刊论文
| 作者 | Zhang, Yan1; Yang, Huirong2; Guo, Xue2; Rong, Naiyan1; Song, Yujiao1; Xu, Youwei2; Lan, Wenxian1; Zhang, Xu3; Liu, Maili3; Xu, Yanhui2 |
| 刊名 | PROTEIN & CELL
 |
| 出版日期 | 2014-11-01 |
| 卷号 | 5期号:11页码:837-850 |
| 关键词 | KDM5B PHD1 H3K4me0 demethylase repression structure |
| 英文摘要 | KDM5B is a histone H3K4me2/3 demethylase. The PHD1 domain of KDM5B is critical for demethylation, but the mechanism underlying the action of this domain is unclear. In this paper, we observed that PHD1(KDM5B) interacts with unmethylated H3K4me0. Our NMR structure of PHD1(KDM5B) in complex with H3K4me0 revealed that the binding mode is slightly different from that of other reported PHD fingers. The disruption of this interaction by double mutations on the residues in the interface (L325A/D328A) decreases the H3K4me2/3 demethylation activity of KDM5B in cells by approximately 50% and increases the transcriptional repression of tumor suppressor genes by approximately twofold. These findings imply that PHD1(KDM5B) may help maintain KDM5B at target genes to mediate the demethylation activities of KDM5B. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Cell Biology |
| 研究领域[WOS] | Cell Biology |
| 关键词[WOS] | STRUCTURAL INSIGHTS ; LYSINE METHYLATION ; TRANSCRIPTIONAL REPRESSION ; SUBSTRATE-SPECIFICITY ; HETERONUCLEAR NMR ; ESCHERICHIA-COLI ; DNA GLYCOSYLASE ; MOLECULAR-BASIS ; JMJD2 FAMILY ; PROTEIN NMR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344867300004 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1418]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 2.Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yan,Yang, Huirong,Guo, Xue,et al. The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B[J]. PROTEIN & CELL,2014,5(11):837-850. |
| APA | Zhang, Yan.,Yang, Huirong.,Guo, Xue.,Rong, Naiyan.,Song, Yujiao.,...&Cao, Chunyang.(2014).The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B.PROTEIN & CELL,5(11),837-850. |
| MLA | Zhang, Yan,et al."The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B".PROTEIN & CELL 5.11(2014):837-850. |
入库方式： OAI收割
来源：武汉物理与数学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。