NMR structures of fusion peptide from influenza hemagglutinin H3 subtype and its mutants
文献类型：期刊论文
| 作者 | Du, Tianpeng1,2; Jiang, Ling1; Liu, Maili1 |
| 刊名 | JOURNAL OF PEPTIDE SCIENCE
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 20期号:4页码:292-297 |
| 关键词 | influenza fusion peptide NMR structure mutation |
| 英文摘要 | The influenza fusion peptide located at the N-terminus of the hemagglutinin HA2 subunit initiates the fusing process of the viral membrane with the host cell endosomal membrane. It had been reported that the structure of a 20-residue H3 subtype fusion peptide (H3-HAfp20) was significantly different with that of a H1 subtype 23-residue one (H1-HAfp23). The sequential difference between the 12th and 15th residues of H1 and H3 subtypes could not fully explain the conformational variation. The first and last three amino acids of H3-HAfp23 involved in formation of hydrogen bonds may play an important role in fusion process. To confirm this hypothesis, we investigate the structures of H3-HAfp23 peptide and its mutants, G1S and G1V, in dodecylphosphatidyl choline micelles by using heteronuclear NMR technology. The results demonstrate that, similar to H1-HAfp23 but significantly different with H3-HAfp20, H3-HAfp23 also has tight helical hairpin structure with the N- and C-terminuses linked together because of the hydrogen bonds between Gly(1) and the last three amino acids, Trp(21)―Tyr(22)―Gly(23). Although the 'hemifusion' G1S and lethal G1V mutants have hairpin-like helical structures, the distances between the N- and C-terminuses are increased as shortage of the hydrogen bonds and the larger kink angle between the antiparallel helices. The paramagnetic ion titration experiments show that the terminuses are inserted into the dodecylphosphatidyl choline micelles used as solving media. These may imply that the tight helical hairpin structure, especially the closed conformation at terminus, plays an important role in fusion activity. Copyright (c) 2014 European Peptide Society and John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | VIRAL MEMBRANE-FUSION ; VIRUS HEMAGGLUTININ ; COILED-COIL ; DOMAIN ; DYNAMICS ; PH ; PROGRAMS ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332973300008 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1431]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst,Wuhan Ctr Magn, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Tianpeng,Jiang, Ling,Liu, Maili. NMR structures of fusion peptide from influenza hemagglutinin H3 subtype and its mutants[J]. JOURNAL OF PEPTIDE SCIENCE,2014,20(4):292-297. |
| APA | Du, Tianpeng,Jiang, Ling,&Liu, Maili.(2014).NMR structures of fusion peptide from influenza hemagglutinin H3 subtype and its mutants.JOURNAL OF PEPTIDE SCIENCE,20(4),292-297. |
| MLA | Du, Tianpeng,et al."NMR structures of fusion peptide from influenza hemagglutinin H3 subtype and its mutants".JOURNAL OF PEPTIDE SCIENCE 20.4(2014):292-297. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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