Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway
文献类型：期刊论文
| 作者 | Yang, Zhongzheng1; Vild, Cody2,3; Ju, Jiaying1; Zhang, Xu4; Liu, Jianping1,5; Shen, Jie1; Zhao, Bin1; Lan, Wenxian1; Gong, Fuchun5; Liu, Maili4 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2012-12-21 |
| 卷号 | 287期号:52页码:43899-43908 |
| 英文摘要 | The AAA-ATPase Vps4 is critical for function of the multivesicular body sorting pathway, which impacts cellular phenomena ranging from receptor down-regulation to viral budding to cytokinesis. Vps4 activity is stimulated by the interaction between Vta1 and Vps60, but the structural basis for this interaction is unclear. The fragment Vps60(128-186) was reported to display the full activity of Vps60. Vta1 interacts with Vps60 using its N-terminal domain (Vta1NTD). In this work, the structure of Vps60(128-186) in complex with Vta1NTD was determined using NMR techniques, demonstrating a novel recognition mode of the microtubule-interacting and transport (MIT) domain in which Vps60(128-186) interacts with Vta1NTD through helices alpha 4' and alpha 5', extending over Vta1NTD MIT2 domain helices 1-3. The Vps60 binding does not result in Vta1 conformational changes, further revealing the fact that Vps4 ATPase is enhanced by the interaction between Vta1 and Vps60 in an unanticipated manner. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MEMBRANE ASSOCIATION ; CHEMICAL-SHIFT ; MACHINERY ; NMR ; BIOGENESIS ; COMPLEXES ; VPS4P ; CYTOKINESIS ; BODIES ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312940800066 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1570]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China 2.Univ Michigan, Sch Med, Inst Life Sci, Ann Arbor, MI 48109 USA 3.Univ Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109 USA 4.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 5.Changsha Univ Sci & Technol, Inst Chem & Biochem Engn, Changsha 410076, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Zhongzheng,Vild, Cody,Ju, Jiaying,et al. Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(52):43899-43908. |
| APA | Yang, Zhongzheng.,Vild, Cody.,Ju, Jiaying.,Zhang, Xu.,Liu, Jianping.,...&Xu, Zhaohui.(2012).Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway.JOURNAL OF BIOLOGICAL CHEMISTRY,287(52),43899-43908. |
| MLA | Yang, Zhongzheng,et al."Structural Basis of Molecular Recognition between ESCRT-III-like Protein Vps60 and AAA-ATPase Regulator Vta1 in the Multivesicular Body Pathway".JOURNAL OF BIOLOGICAL CHEMISTRY 287.52(2012):43899-43908. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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