Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp
文献类型：期刊论文
| 作者 | Qin, Xiaorong1; Zhang, Mingjin2; Qin, Jian1; Yuan, Shiwei1; Hou, Yali1; Liu, Jianzhong1 |
| 刊名 | SEPARATION AND PURIFICATION TECHNOLOGY
 |
| 出版日期 | 2012-03-05 |
| 卷号 | 87页码:79-83 |
| 关键词 | Heat treatment Ammonium sulfate precipitation Immobilized metal affinity chromatography Superoxide dismutase Glycoprotein |
| 英文摘要 | Copper and zinc containing superoxide dismutase (Cu,Zn-SOD) from pumpkin (Cucurbita moschata) stringy pulp was purified in two steps. The process included the combination of heat treatment (55 degrees C, 45 min) with ammonium sulfate (10%) precipitation and immobilized metal affinity chromatography, yielding a 87-fold purification (activity recovery of 42%) and a single band upon SDS-PAGE. The enzyme had a molecular mass of 35.0 kDa, as determined by gel filtration chromatography and 2884 U mg(-1) protein-specific activity. It was a dimeric enzyme with two identical subunits of 17,477 Da, as measured using MicrOTOF-Q mass spectrometer and each subunit had a Cu and Zn element. Pumpkin pulp Cu,Zn-SOD is a novel glycosylated enzyme, which possesses higher molecular mass and wider range of pH dependence of the activity than other plant Cu,Zn-SOD. This naturally glycoprotein remained its activity at pH 4-12 at 25 degrees C for 2 h and the enzyme remained its activity lower than 50 degrees C at pH 7.8 for 45 min. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | CONTAINING SUPEROXIDE-DISMUTASE ; BINDING ; FUNGAL ; TISSUE ; LEAVES ; CU |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302204300010 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1624]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Wuhan Univ Sci & Technol, Coll Chem Engn & Technol, Wuhan 430081, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Xiaorong,Zhang, Mingjin,Qin, Jian,et al. Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2012,87:79-83. |
| APA | Qin, Xiaorong,Zhang, Mingjin,Qin, Jian,Yuan, Shiwei,Hou, Yali,&Liu, Jianzhong.(2012).Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp.SEPARATION AND PURIFICATION TECHNOLOGY,87,79-83. |
| MLA | Qin, Xiaorong,et al."Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp".SEPARATION AND PURIFICATION TECHNOLOGY 87(2012):79-83. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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