中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp

文献类型:期刊论文

作者Qin, Xiaorong1; Zhang, Mingjin2; Qin, Jian1; Yuan, Shiwei1; Hou, Yali1; Liu, Jianzhong1
刊名SEPARATION AND PURIFICATION TECHNOLOGY
出版日期2012-03-05
卷号87页码:79-83
关键词Heat treatment Ammonium sulfate precipitation Immobilized metal affinity chromatography Superoxide dismutase Glycoprotein
英文摘要Copper and zinc containing superoxide dismutase (Cu,Zn-SOD) from pumpkin (Cucurbita moschata) stringy pulp was purified in two steps. The process included the combination of heat treatment (55 degrees C, 45 min) with ammonium sulfate (10%) precipitation and immobilized metal affinity chromatography, yielding a 87-fold purification (activity recovery of 42%) and a single band upon SDS-PAGE. The enzyme had a molecular mass of 35.0 kDa, as determined by gel filtration chromatography and 2884 U mg(-1) protein-specific activity. It was a dimeric enzyme with two identical subunits of 17,477 Da, as measured using MicrOTOF-Q mass spectrometer and each subunit had a Cu and Zn element. Pumpkin pulp Cu,Zn-SOD is a novel glycosylated enzyme, which possesses higher molecular mass and wider range of pH dependence of the activity than other plant Cu,Zn-SOD. This naturally glycoprotein remained its activity at pH 4-12 at 25 degrees C for 2 h and the enzyme remained its activity lower than 50 degrees C at pH 7.8 for 45 min. (C) 2011 Elsevier B.V. All rights reserved.
WOS标题词Science & Technology ; Technology
类目[WOS]Engineering, Chemical
研究领域[WOS]Engineering
关键词[WOS]CONTAINING SUPEROXIDE-DISMUTASE ; BINDING ; FUNGAL ; TISSUE ; LEAVES ; CU
收录类别SCI
语种英语
WOS记录号WOS:000302204300010
公开日期2015-07-14
源URL[http://ir.wipm.ac.cn/handle/112942/1624]  
专题武汉物理与数学研究所_磁共振基础研究部
作者单位1.Wuhan Univ Sci & Technol, Coll Chem Engn & Technol, Wuhan 430081, Hubei, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
推荐引用方式
GB/T 7714
Qin, Xiaorong,Zhang, Mingjin,Qin, Jian,et al. Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2012,87:79-83.
APA Qin, Xiaorong,Zhang, Mingjin,Qin, Jian,Yuan, Shiwei,Hou, Yali,&Liu, Jianzhong.(2012).Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp.SEPARATION AND PURIFICATION TECHNOLOGY,87,79-83.
MLA Qin, Xiaorong,et al."Two-step purification of Cu,Zn-superoxide dismutase from pumpkin (Cucurbita moschata) pulp".SEPARATION AND PURIFICATION TECHNOLOGY 87(2012):79-83.

入库方式: OAI收割

来源:武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。