Purification and characterization of Cu,Zn superoxide dismutase from pumpkin (Cucurbita moschata) pulp
文献类型：期刊论文
| 作者 | Qin, Xiaorong1; Zhang, Mingjin2; Wu, Lin1 |
| 刊名 | EUROPEAN FOOD RESEARCH AND TECHNOLOGY
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 235期号:6页码:1049-1054 |
| 关键词 | Cucurbita moschata Pumpkin Pulp Superoxide dismutase Chromatography |
| 英文摘要 | Superoxide dismutase (SOD, EC 1.15.1.1) plays an important role in pharmaceutical, neutraceutical and cosmeceutical industries as it can eliminate toxic superoxide radicals. Copper and zinc SOD was purified from pumpkin (Cucurbita moschata) stringy pulp. The stepwise procedure consisted phosphate extraction, heat treatment, ammonium sulfate precipitation, Sephadex G-100 gel filtration and DEAE-52 chromatography. This procedure resulted in a single band on native polyacrylamide gels and sodium dodecyl sulfate-polyacrylamide gels. The enzyme was purified 54.4-fold with its specific activity of 1,794 U/mg protein and the yield of 23.1 %. The enzyme molecular mass was estimated at 35.3 +/- A 0.6 kDa by gel filtration. The subunit molecular mass of this homo-dimeric protein was estimated at 17.5 +/- A 0.3 kDa on sodium dodecyl sulfate-polyacrylamide gels. The purified enzyme, which was characterized as a H2O2-sensitive SOD, contained approximately one copper atom and one zinc atom per subunit, indicating that it belongs to the Cu,Zn-SOD. The enzyme was found to remain thermostable lower than 50 A degrees C. Pumpkin pulp Cu,Zn-SOD appeared to remain wider range of pH stability than other plant Cu,Zn-SOD, as it showed a strong and steady activity at a wider range of pH from 4 to 12 at 25 A degrees C for 2 h. The enzyme was slightly inhibited by SDS and NaN3, but was not inhibited by Na3BO3 and sodium citrate. Therefore, these results suggested that pumpkin stringy pulp which is considered as a waste can be used as a rich source of Cu,Zn-SOD and can be commercially exploited in cosmeceuticals, pharmaceuticals and neutraceuticals. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Food Science & Technology |
| 研究领域[WOS] | Food Science & Technology |
| 关键词[WOS] | DISEASE ; LEAVES ; L. ; CU |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311392100007 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1654]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Wuhan Univ Sci & Technol, Coll Chem Engn & Technol, Wuhan 430081, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Xiaorong,Zhang, Mingjin,Wu, Lin. Purification and characterization of Cu,Zn superoxide dismutase from pumpkin (Cucurbita moschata) pulp[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2012,235(6):1049-1054. |
| APA | Qin, Xiaorong,Zhang, Mingjin,&Wu, Lin.(2012).Purification and characterization of Cu,Zn superoxide dismutase from pumpkin (Cucurbita moschata) pulp.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,235(6),1049-1054. |
| MLA | Qin, Xiaorong,et al."Purification and characterization of Cu,Zn superoxide dismutase from pumpkin (Cucurbita moschata) pulp".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 235.6(2012):1049-1054. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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