Fast detection of choline-containing metabolites in liver using 2D H-1-N-14 three-bond correlation (HN3BC) spectroscopy
文献类型：期刊论文
| 作者 | Mao, Xi-an1,2; Li, Ning3; Mao, Jiezhen2; Li, Qiurong3; Xiao, Nan2; Jiang, Bin2 ; Jiang, Ling2; Wang, Xu-xia2; Liu, Maili2
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| 刊名 | JOURNAL OF MAGNETIC RESONANCE
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| 出版日期 | 2012 |
| 卷号 | 214页码:352-359 |
| 关键词 | Choline-containing metabolites Liver Carcinoma tissue Fast detection H-1-N-14 three-bond correlation spectroscopy |
| 英文摘要 | Detection and quantification of total choline-containing metabolites (CCMs) in tissues by magnetic resonance spectroscopy (MRS) has received considerable attention as a biomarker of cancer. Tissue CCMs are mainly choline (Cho), phosphocholine (PCho), and glycerophosphocholine (GPCho). Because the methyl H-1 resonances of tissue CCMs exhibit small chemical shift differences and overlap significantly in 19 H-1 MRS, quantification of individual components is precluded. Development of a MRS method capably of resolving individual components of tissue CCMs would be a significant advance. Herein, a modification of the 2D H-1-N-14 HSQC technique is targeted on the two methylene H-1 in the CH2O group ((3)J(1H14N) = 2.7 Hz) and applied to ex vivo mouse and human liver samples at physiological temperature (37 degrees C). Specifically, the H-1-N-14 HSQC technique is modified into a 2D H-1-N-14 three-bond correlation (HN3BC) experiment, which selectively detects the H-1 of CH2O coupled to N-14 in CCMs. Separate signals from Cho, PCho, and GPCho components are resolved with high detection sensitivity. A 2D HN3BC spectrum can be recorded from mouse liver in only 1.5 min and from human carcinoma liver tissue in less than 3 min with effective sample volume of 0.2 ml at 14.1 T. (C) 2011 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biochemical Research Methods ; Physics, Atomic, Molecular & Chemical ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Physics ; Spectroscopy |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; N-14 NMR-SPECTROSCOPY ; PHOSPHOLIPID-METABOLISM ; CLINICAL-APPLICATIONS ; CANCER ; PROTON ; TARGET ; TUMORS ; CELLS ; MRS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000299656400047 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1688]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan 430071, Hubei, Peoples R China 3.Jinling Hosp, Res Inst Gen Surg, Nanjing 210002, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Xi-an,Li, Ning,Mao, Jiezhen,et al. Fast detection of choline-containing metabolites in liver using 2D H-1-N-14 three-bond correlation (HN3BC) spectroscopy[J]. JOURNAL OF MAGNETIC RESONANCE,2012,214:352-359. |
| APA | Mao, Xi-an.,Li, Ning.,Mao, Jiezhen.,Li, Qiurong.,Xiao, Nan.,...&Liu, Maili.(2012).Fast detection of choline-containing metabolites in liver using 2D H-1-N-14 three-bond correlation (HN3BC) spectroscopy.JOURNAL OF MAGNETIC RESONANCE,214,352-359. |
| MLA | Mao, Xi-an,et al."Fast detection of choline-containing metabolites in liver using 2D H-1-N-14 three-bond correlation (HN3BC) spectroscopy".JOURNAL OF MAGNETIC RESONANCE 214(2012):352-359. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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