Dual-band selective double cross polarization for heteronuclear polarization transfer between dilute spins in solid-state MAS NMR
文献类型：期刊论文
| 作者 | Zhang, Zhengfeng1; Miao, Yimin2,3; Liu, Xiaoli1; Yang, Jun1; Li, Conggang1; Deng, Feng1; Fu, Riqiang3 |
| 刊名 | JOURNAL OF MAGNETIC RESONANCE
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 217页码:92-99 |
| 关键词 | Double cross polarization (DCP) NCA/NCO Solid-state MAS NMR Heteronuclear polarization transfer |
| 英文摘要 | A sinusoidal modulation scheme is described for selective heteronuclear polarization transfer between two dilute spins in double cross polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. During the second N -> C cross polarization, the C-13 RF amplitude is modulated sinusoidally while the N-15 RF amplitude is tangent. This modulation induces an effective spin-lock field in two selective frequency bands in either side of the C-13 RF carrier frequency, allowing for simultaneous polarization transfers from N-15 to C-13 in those two selective frequency bands. It is shown by experiments and simulations that this sinusoidal modulation allows one to selectively polarize from N-15 to its covalently bonded C-13 alpha and C-13' carbons in neighboring peptide planes simultaneously, which is useful for establishing the backbone connectivity between two sequential residues in protein structural elucidation. The selectivity and efficiency were experimentally demonstrated on a uniformly C-13,N-15-labeled beta 1 immunoglobulin binding domain of protein G (GB1). (C) 2012 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology |
| 类目[WOS] | Biochemical Research Methods ; Physics, Atomic, Molecular & Chemical ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Physics ; Spectroscopy |
| 关键词[WOS] | ANGLE-SPINNING NMR ; NUCLEAR-MAGNETIC-RESONANCE ; SHIFT CORRELATION SPECTROSCOPY ; CHEMICAL-SHIFT ; ROTATING SOLIDS ; SYMMETRY PRINCIPLES ; COHERENCE TRANSFER ; PULSE SEQUENCES ; ASSIGNMENT ; PHASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303083500013 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1730]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA 3.Natl High Magnet Field Lab, Tallahassee, FL 32310 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Zhengfeng,Miao, Yimin,Liu, Xiaoli,et al. Dual-band selective double cross polarization for heteronuclear polarization transfer between dilute spins in solid-state MAS NMR[J]. JOURNAL OF MAGNETIC RESONANCE,2012,217:92-99. |
| APA | Zhang, Zhengfeng.,Miao, Yimin.,Liu, Xiaoli.,Yang, Jun.,Li, Conggang.,...&Fu, Riqiang.(2012).Dual-band selective double cross polarization for heteronuclear polarization transfer between dilute spins in solid-state MAS NMR.JOURNAL OF MAGNETIC RESONANCE,217,92-99. |
| MLA | Zhang, Zhengfeng,et al."Dual-band selective double cross polarization for heteronuclear polarization transfer between dilute spins in solid-state MAS NMR".JOURNAL OF MAGNETIC RESONANCE 217(2012):92-99. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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