Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy
文献类型：期刊论文
| 作者 | Li, Huihui1; An, Yanpeng1,2; Zhang, Lulu1; Lei, Hehua1; Zhang, Limin1; Wang, Yulan1,3; Tang, Huiru1,2 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2013-12-01 |
| 卷号 | 12期号:12页码:5520-5534 |
| 关键词 | carrageenan-injection pleurisy inflammation metabonomics fatty acids |
| 英文摘要 | Inflammation is closely associated with pathogenesis of various metabolic disorders, cardiovascular diseases, and cancers. To understand the systems responses to localized inflammation, we analyzed the dynamic metabolic changes in rat plasma and urine associated with the carrageenan-induced self-limiting pleurisy using NMR spectroscopy in conjunction with multivariate data analysis. Fatty acids in plasma were also analyzed using GC-FID/MS with the data from clinical chemistry and histopathology as complementary information. We found that in the acute phase of inflammation rats with pleurisy had significantly lower levels in serum albumin, fatty acids, and lipoproteins but higher globulin level and larger quantity of pleural exudate than controls. The carrageenan-induced inflammation was accompanied by significant metabolic alterations involving TCA cycle, glycolysis, biosyntheses of acute phase proteins, and metabolisms of amino acids, fatty acids, ketone bodies, and choline in acute phase. The resolution process of pleurisy was heterogeneous, and two subgroups were observed for the inflammatory rats at day-6 post treatment with different metabolic features together with the quantity of pleural exudate and weights of thymus and spleen. The metabolic differences between these subgroups were reflected in the levels of albumin and acute-phase proteins, the degree of returning to normality for multiple metabolic pathways including glycolysis, TCA cycle, gut microbiota functions, and metabolisms of lipids, choline and vitamin B-3. These findings provided some essential details for the dynamic metabolic changes associated with the carrageenan-induced self-limiting inflammation and demonstrated the combined NMR and GC-FID/MS analysis as a powerful approach for understanding biochemical aspects of inflammation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ACUTE-PHASE RESPONSE ; SPINNING H-1-NMR SPECTROSCOPY ; POLYUNSATURATED FATTY-ACIDS ; MULTIVARIATE DATA-ANALYSIS ; NITRIC-OXIDE SYNTHASE ; HUMAN-BLOOD-PLASMA ; ACUTE-INFLAMMATION ; GUT MICROBIOTA ; RESOLUTION ; CYCLOOXYGENASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000328231300015 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/786]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Univ Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn Biospect & Metabol, Shanghai 200433, Peoples R China 3.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310058, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Huihui,An, Yanpeng,Zhang, Lulu,et al. Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(12):5520-5534. |
| APA | Li, Huihui.,An, Yanpeng.,Zhang, Lulu.,Lei, Hehua.,Zhang, Limin.,...&Tang, Huiru.(2013).Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy.JOURNAL OF PROTEOME RESEARCH,12(12),5520-5534. |
| MLA | Li, Huihui,et al."Combined NMR and GC-MS Analyses Revealed Dynamic Metabolic Changes Associated with the Carrageenan-Induced Rat Pleurisy".JOURNAL OF PROTEOME RESEARCH 12.12(2013):5520-5534. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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