Cerebral Ischemia Is Exacerbated by Extracellular Nicotinamide Phosphoribosyltransferase via a Non-Enzymatic Mechanism
文献类型：期刊论文
| 作者 | Zhao, Bing1,4; Zhang, Meng3; Han, Xue1; Zhang, Xia-Yan1; Xing, Qiong2; Dong, Xu2; Shi, Qiao-Juan1; Huang, Peng1; Lu, Yun-Bi1; Wei, Er-Qing1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-12-31 |
| 卷号 | 8期号:12 |
| 英文摘要 | Intracellular nicotinamide phosphoribosyltransferase (iNAMPT) in neuron has been known as a protective factor against cerebral ischemia through its enzymatic activity, but the role of central extracellular NAMPT (eNAMPT) is not clear. Here we show that eNAMPT protein level was elevated in the ischemic rat brain after middle-cerebral-artery occlusion (MCAO) and reperfusion, which can be traced to at least in part from blood circulation. Administration of recombinant NAMPT protein exacerbated MCAO-induced neuronal injury in rat brain, while exacerbated oxygen-glucose-deprivation (OGD) induced neuronal injury only in neuron-glial mixed culture, but not in neuron culture. In the mixed culture, NAMPT protein promoted TNF-alpha release in a time-and concentration-dependent fashion, while TNF-alpha neutralizing antibody protected OGD-induced, NAMPT-enhanced neuronal injury. Importantly, H247A mutant of NAMPT with essentially no enzymatic activity exerted similar effects on ischemic neuronal injury and TNF-alpha release as the wild type protein. Thus, eNAMPT is an injurious and inflammatory factor in cerebral ischemia and aggravates ischemic neuronal injury by triggering TNF-alpha release from glia cells, via a mechanism not related to NAMPT enzymatic activity. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | COLONY-ENHANCING FACTOR ; VISFATIN CONCENTRATIONS ; STROKE ; MODEL ; NEUROPROTECTION ; EXPRESSION ; PROTECTS ; DISEASE ; NAD(+) ; BRAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329323900151 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/832]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Zhejiang Univ, Sch Med, Dept Pharmacol,Zhejiang Prov Key Lab Neurobiol, Key Lab Med Neurobiol,Minist Hlth China, Hangzhou 310003, Zhejiang, Peoples R China 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet, Wuhan Inst Phys & Math, Wuhan, Peoples R China 3.Zhejiang Univ, Sch Med, Dept Physiol, Hangzhou 310003, Zhejiang, Peoples R China 4.Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Anesthesiol, Hangzhou 310003, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Bing,Zhang, Meng,Han, Xue,et al. Cerebral Ischemia Is Exacerbated by Extracellular Nicotinamide Phosphoribosyltransferase via a Non-Enzymatic Mechanism[J]. PLOS ONE,2013,8(12). |
| APA | Zhao, Bing.,Zhang, Meng.,Han, Xue.,Zhang, Xia-Yan.,Xing, Qiong.,...&Tang, Chun.(2013).Cerebral Ischemia Is Exacerbated by Extracellular Nicotinamide Phosphoribosyltransferase via a Non-Enzymatic Mechanism.PLOS ONE,8(12). |
| MLA | Zhao, Bing,et al."Cerebral Ischemia Is Exacerbated by Extracellular Nicotinamide Phosphoribosyltransferase via a Non-Enzymatic Mechanism".PLOS ONE 8.12(2013). |
入库方式： OAI收割
来源：武汉物理与数学研究所
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