Enhanced Green Fluorescent Protein Transgenic Expression In Vivo Is Not Biologically Inert
文献类型：期刊论文
| 作者 | Li, Hongde1,3; Wei, Hong2; Wang, Yong2; Tang, Huiru1; Wang, Yulan1,4 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2013-08-01 |
| 卷号 | 12期号:8页码:3801-3808 |
| 关键词 | 3-ureidopropionate EGFP metabolism metabonomics transgene |
| 英文摘要 | Enhanced green fluorescent protein (EGFP) is a widely used biological reporter. However, the effects of EGFP expression in vivo are still unclear. To investigate the effects of EGFP transgenic expression in vivo, we employed an NMR-based metabonomics method to analyze the metabonome of EGFP transgenic mice. The results show that the metabonomes of urine, liver, and kidney of the EGFP transgenic mice are different from their wild-type counterparts. The EGFP mice expressed high levels of urinary 3-ureidopropionate, which is due to the down-regulated transcriptional level of beta-ureidopropionase. The expression of EGFP in vivo also affects other metabolic pathways, including nucleic acid metabolism, energy utilization, and amino acids catabolism. These findings indicate that EGFP transgenic expression is not as inert as has been considered. Our investigation provides a holistic view on the effect of EGFP expression in vivo, which is useful when EGFP is employed as a functional biological indicator. Our work also highlights the potential of a metabonomics strategy in studying the association between molecular phenotypes and gene function. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MANNOSE-BINDING LECTIN ; METABOLIC PHENOTYPES ; PYRUVATE-KINASE ; MOUSE ; RESPONSES ; MICE ; NMR ; SYSTEMS ; TRANSDUCTION ; SPECTROSCOPY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322852800023 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/902]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Third Mil Med Univ, Dept Lab Anim Sci, Chongqing 400038, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hongde,Wei, Hong,Wang, Yong,et al. Enhanced Green Fluorescent Protein Transgenic Expression In Vivo Is Not Biologically Inert[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(8):3801-3808. |
| APA | Li, Hongde,Wei, Hong,Wang, Yong,Tang, Huiru,&Wang, Yulan.(2013).Enhanced Green Fluorescent Protein Transgenic Expression In Vivo Is Not Biologically Inert.JOURNAL OF PROTEOME RESEARCH,12(8),3801-3808. |
| MLA | Li, Hongde,et al."Enhanced Green Fluorescent Protein Transgenic Expression In Vivo Is Not Biologically Inert".JOURNAL OF PROTEOME RESEARCH 12.8(2013):3801-3808. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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