High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats
文献类型：期刊论文
| 作者 | An, Yanpeng1,3; Xu, Wenxin1,3; Li, Huihui1,3; Lei, Hehua1; Zhang, Limin1; Hao, Fuhua1 ; Duan, Yixuan1,4; Yan, Xing1,3; Zhao, Ying1,4; Wu, Junfang1
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2013-08-01 |
| 卷号 | 12期号:8页码:3755-3768 |
| 关键词 | obesity metabolism high-fat diet NMR multivariate data analysis |
| 英文摘要 | Obesity is a condition resulting from the interactions of individual biology and environmental factors causing multiple complications. To understand the system's metabolic changes associated with the obesity development and progression, we systematically analyzed the dynamic metabonomic changes induced by a high-fat diet (HFD) in multiple biological matrices of rats using NMR and GC-FID/MS techniques. Clinical chemistry and histopathological data were obtained as complementary information. We found that HFD intakes caused systematic metabolic changes in blood plasma, liver, and urine samples involving multiple metabolic pathways including glycolysis, TCA cycle, and gut microbiota functions together with the metabolisms of fatty adds, amino adds, choline, B-vitamins, purines, and pyrimidines. The HFD-induced metabolic variations were detectable in rat urine a week after HFD intake and showed clear dependence on the intake duration. B-vitamins and gut microbiota played important roles in the obesity development and progression together with changes in TCA cycle intermediates (citrate, a-ketoglutarate, succinate, and fumarate). 83-day HFD intakes caused significant metabolic alterations in rat liver highlighted with the enhancements in lipogenesis, lipid accumulation and lipid oxidation, suppression of glycolysis, up-regulation of gluconeogenesis and glycogenesis together with altered metabolisms of choline, amino acids and nucleotides. HFD intakes reduced the PUFA-to-MUFA ratio in both plasma and liver, indicating the HFD-induced oxidative stress. These findings provided essential biochemistry information about the dynamic metabolic responses to the development and progression of HFD-induced obesity. This study also demonstrated the combined metabonomic analysis of multiple biological matrices as a powerful approach for understanding the molecular basis of pathogenesis and disease progression. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | INSULIN-RESISTANT MICE ; HUMAN-BLOOD-PLASMA ; H-1-NMR SPECTROSCOPY ; GUT MICROBIOTA ; OBESITY ; LIVER ; ACID ; SYSTEMS ; STRESS ; METABONOMICS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322852800019 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1034]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Yanpeng,Xu, Wenxin,Li, Huihui,et al. High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(8):3755-3768. |
| APA | An, Yanpeng.,Xu, Wenxin.,Li, Huihui.,Lei, Hehua.,Zhang, Limin.,...&Tang, Huiru.(2013).High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats.JOURNAL OF PROTEOME RESEARCH,12(8),3755-3768. |
| MLA | An, Yanpeng,et al."High-Fat Diet Induces Dynamic Metabolic Alterations in Multiple Biological Matrices of Rats".JOURNAL OF PROTEOME RESEARCH 12.8(2013):3755-3768. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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