Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis
文献类型：期刊论文
| 作者 | Zhao, Hong1; Chiaro, Christopher R.2; Zhang, Limin2,4; Smith, Philip B.2; Chan, Chung Yu3; Pedley, Anthony M.1; Pugh, Raymond J.1; French, Jarrod B.5,6; Patterson, Andrew D.2; Benkovic, Stephen J.1 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2015-03-13 |
| 卷号 | 290期号:11页码:6705-6713 |
| 英文摘要 | Enzymes in the de novo purine biosynthesis pathway are recruited to form a dynamic metabolic complex referred to as the purinosome. Previous studies have demonstrated that purinosome assembly responds to purine levels in culture medium. Purine-depleted medium or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole (DMAT) treatment stimulates the purinosome assembly in HeLa cells. Here, several metabolomic technologies were applied to quantify the static cellular levels of purine nucleotides and measure the de novo biosynthesis rate of IMP, AMP, and GMP. Direct comparison of purinosome-rich cells (cultured in purine-depleted medium) and normal cells showed a 3-fold increase in IMP concentration in purinosomerich cells and similar levels of AMP, GMP, and ratios of AMP/GMP and ATP/ADP for both. In addition, a higher level of IMP was also observed in HeLa cells treated with DMAT. Furthermore, increases in the de novo IMP/AMP/GMP biosynthetic flux rate under purine-depleted condition were observed. The synthetic enzymes, adenylosuccinate synthase (ADSS) and inosine monophosphate dehydrogenase (IMPDH), downstream of IMP were also shown to be part of the purinosome. Collectively, these results provide further evidence that purinosome assembly is directly related to activated de novo purine biosynthesis, consistent with the functionality of the purinosome. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ADENYLOSUCCINATE SYNTHETASE ; MASS-SPECTROMETRY ; CELL-GROWTH ; ENZYME ; PURIFICATION ; COMPLEX ; DNA ; COMPARTMENTATION ; VARIANTS ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350991500005 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1051]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Penn State Univ, Dept Chem, University Pk, PA 16802 USA 2.Penn State Univ, Metabol Facil, Ctr Mol Toxicol & Carcinogenesis, University Pk, PA 16802 USA 3.Penn State Univ, Dept Engn Sci & Mech, University Pk, PA 16802 USA 4.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 5.SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA 6.SUNY Stony Brook, Dept Chem, Stony Brook, NY 11794 USA |
| 推荐引用方式 GB/T 7714 | Zhao, Hong,Chiaro, Christopher R.,Zhang, Limin,et al. Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015,290(11):6705-6713. |
| APA | Zhao, Hong.,Chiaro, Christopher R..,Zhang, Limin.,Smith, Philip B..,Chan, Chung Yu.,...&Benkovic, Stephen J..(2015).Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis.JOURNAL OF BIOLOGICAL CHEMISTRY,290(11),6705-6713. |
| MLA | Zhao, Hong,et al."Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis".JOURNAL OF BIOLOGICAL CHEMISTRY 290.11(2015):6705-6713. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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