Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice
文献类型：期刊论文
| 作者 | Wang, Zhaohai1; Wang, Ya1; Hong, Xiao1; Hu, Daoheng1; Liu, Caixiang2; Yang, Jing1; Li, Yang1; Huang, Yunqing3; Feng, Yuqi3; Gong, Hanyu1 |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2015-02-01 |
| 卷号 | 66期号:3页码:973-987 |
| 关键词 | Defence responses leaf senescence reactive oxygen species (ROS) rice (Oryza sativa) SPL29 UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) |
| 英文摘要 | Plant leaf senescence and defence responses are important biological processes, but the molecular mechanisms involved are not well understood. This study identified a new rice mutant, spotted leaf 29 (spl29). The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis. The mutant spl29 lacks UAP activity. The biological phenotypes for which UAP is responsible have not previously been reported in plants. The spl29 mutant displayed early leaf senescence, confirmed by chlorophyll loss and photosystem II decline as physiological indicators, chloroplast degradation as a cellular characteristic, and both upregulation of senescence transcription factors and senescence-associated genes, and downregulation of photosynthesis-related genes, as molecular evidence. Defence responses were induced in the spl29 mutant, shown by enhanced resistance to bacterial blight inoculation and upregulation of defence response genes. Reactive oxygen species, including O-2(-) and H2O2, accumulated in spl29 plants; there was also increased malondialdehyde content. Enhanced superoxide dismutase activity combined with normal catalase activity in spl29 could be responsible for H2O2 accumulation. The plant hormones jasmonic acid and abscisic acid also accumulated in spl29 plants. ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant. Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | LESION MIMIC MUTANTS ; DISEASE RESISTANCE ; SIGNAL-TRANSDUCTION ; OXIDATIVE STRESS ; CELL-DEATH ; GENE ; ARABIDOPSIS ; EXPRESSION ; IDENTIFICATION ; DEGRADATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351662300029 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1056]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Wuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance,Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 3.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ, Dept Chem, Wuhan 430072, Hubei, Peoples R China 4.Fudan Univ, State Key Lab Genet Engn, Metabol Lab, Sch Life Sci, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhaohai,Wang, Ya,Hong, Xiao,et al. Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(3):973-987. |
| APA | Wang, Zhaohai.,Wang, Ya.,Hong, Xiao.,Hu, Daoheng.,Liu, Caixiang.,...&Li, Yangsheng.(2015).Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice.JOURNAL OF EXPERIMENTAL BOTANY,66(3),973-987. |
| MLA | Wang, Zhaohai,et al."Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice".JOURNAL OF EXPERIMENTAL BOTANY 66.3(2015):973-987. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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