Visualizing an Ultra-Weak Protein-Protein Interaction in Phosphorylation Signaling
文献类型：期刊论文
| 作者 | Xing, Qiong3; Huang, Peng1,2; Yang, Ju3; Sun, Jian-Qiang3; Gong, Zhou3; Dong, Xu3; Guo, Da-Chuan3; Chen, Shao-Min3; Yang, Yu-Hong3; Wang, Yan3 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2014-10-20 |
| 卷号 | 53期号:43页码:11501-11505 |
| 关键词 | NMR spectroscopy paramagnetic relaxation enhancement proteins protein-protein interactions signal transduction |
| 英文摘要 | Proteins interact with each other to fulfill their functions. The importance of weak protein-protein interactions has been increasingly recognized. However, owing to technical difficulties, ultra-weak interactions remain to be characterized. Phosphorylation can take place via a K-D approximate to 25mM interaction between two bacterial enzymes. Using paramagnetic NMR spectroscopy and with the introduction of a novel Gd-III-based probe, we determined the structure of the resulting complex to atomic resolution. The structure accounts for the mechanism of phosphoryl transfer between the two enzymes and demonstrates the physical basis for their ultra-weak interaction. Further, molecular dynamics (MD) simulations suggest that the complex has a lifetime in the micro- to millisecond regimen. Hence such interaction is termed a fleeting interaction. From mathematical modeling, we propose that an ultra-weak fleeting interaction enables rapid flux of phosphoryl signal, providing a high effective protein concentration. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ESCHERICHIA-COLI ; PHOSPHOTRANSFERASE SYSTEM ; PARAMAGNETIC NMR ; TRANSFER COMPLEX ; TERMINAL DOMAIN ; ENZYME I ; PHOSPHOENOLPYRUVATE ; SIMULATION ; DYNAMICS ; BACTERIA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343870400015 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1317]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Zhejiang Univ, Sch Med, Dept Pharmacol, Hangzhou 310028, Zhejiang, Peoples R China 2.Zhejiang Univ, Sch Med, Inst Neurosci, Hangzhou 310028, Zhejiang, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom Mol Phys, CAS Key Lab Magnet Resonance Biol Syst,Wuhan Ctr, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xing, Qiong,Huang, Peng,Yang, Ju,et al. Visualizing an Ultra-Weak Protein-Protein Interaction in Phosphorylation Signaling[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2014,53(43):11501-11505. |
| APA | Xing, Qiong.,Huang, Peng.,Yang, Ju.,Sun, Jian-Qiang.,Gong, Zhou.,...&Tang, Chun.(2014).Visualizing an Ultra-Weak Protein-Protein Interaction in Phosphorylation Signaling.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,53(43),11501-11505. |
| MLA | Xing, Qiong,et al."Visualizing an Ultra-Weak Protein-Protein Interaction in Phosphorylation Signaling".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53.43(2014):11501-11505. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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