Dynamic metabolomic responses of Escherichia coli to nicotine stress
文献类型：期刊论文
| 作者 | Ding, Lijian1; Chen, Juanjuan1; Zou, Jianding2; Zhang, Limin3; Ye, Yangfang1 |
| 刊名 | CANADIAN JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 60期号:8页码:547-556 |
| 关键词 | nicotine Escherichia coli metabolomics nuclear magnetic resonance (NMR) multivariate data analysis |
| 英文摘要 | Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolomics in combination with multivariate data analysis methods to comprehensively analyze the metabolic changes in nicotine-treated Escherichia coli. Our results showed that nicotine caused the changes of energy-related metabolism that we believe are due to enhanced glycolysis and mixed acid fermentation as well as inhibited tricarboxylic acid cycle activity. Furthermore, nicotine resulted in the alteration of choline metabolism with a decreased synthesis of betaine but an increased production of dimethylamine. Moreover, nicotine caused a decrease in amino acid concentration and an alteration of nucleotide synthesis. We hypothesize that these changes caused the decrease in bacterial cell density observed in the experiment. These findings provide a comprehensive insight into the metabolic response of E. coli to nicotine stress. Our study highlights the value of metabolomics in elucidating the metabolic mechanisms of nicotine action. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology |
| 关键词[WOS] | GLYCINE BETAINE PATHWAY ; NMR-BASED METABOLOMICS ; OXIDATIVE STRESS ; CANCER-CELLS ; EXPRESSION ; PROTEINS ; INDICATORS ; APOPTOSIS ; RELEASE ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341918300007 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1352]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Cixi Peoples Hosp, Cixi 315300, Zhejiang, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Lijian,Chen, Juanjuan,Zou, Jianding,et al. Dynamic metabolomic responses of Escherichia coli to nicotine stress[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2014,60(8):547-556. |
| APA | Ding, Lijian,Chen, Juanjuan,Zou, Jianding,Zhang, Limin,&Ye, Yangfang.(2014).Dynamic metabolomic responses of Escherichia coli to nicotine stress.CANADIAN JOURNAL OF MICROBIOLOGY,60(8),547-556. |
| MLA | Ding, Lijian,et al."Dynamic metabolomic responses of Escherichia coli to nicotine stress".CANADIAN JOURNAL OF MICROBIOLOGY 60.8(2014):547-556. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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