A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement
文献类型：期刊论文
| 作者 | Gu, Xin-Hua1,2; Gong, Zhou1,2; Guo, Da-Chuan1,2; Zhang, Wei-Ping3; Tang, Chun1,2 |
| 刊名 | JOURNAL OF BIOMOLECULAR NMR
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 58期号:3页码:149-154 |
| 关键词 | NMR spectroscopy Protein structures Paramagnetic relaxation enhancement Paramagnetic probe Solvent PRE |
| 英文摘要 | Solvent paramagnetic relaxation enhancement (sPRE) arises from random collisions between paramagnetic cosolvent and protein of interest. The sPRE can be readily measured, affording protein structure information. However, lack of an inert cosolvent probe may yield sPRE values that are not consistent with protein structure. Here we synthesized a new sPRE probe, triethylenetetraamine hexaacetate trimethylamide gadolinium, or Gd(III)-TTHA-TMA. With a total of 10 coordination sites, this paramagnetic cosovlent eliminates an inner-sphere water molecule that can otherwise transfer relaxation to protein through exchange. With the metal ion centered, the new probe is largely spherical with a radius of 4.0 , permitting accurate back calculation of sPRE. The effectiveness Gd(III)-TTHA-TMA as a sPRE probe was demonstrated on three well-studied protein systems. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Spectroscopy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Spectroscopy |
| 关键词[WOS] | NMR-SPECTROSCOPY ; PRACTICAL ASPECTS ; COMPLEXES ; MACROMOLECULES ; IDENTIFICATION ; RECOGNITION ; SURFACES ; MOLECULE ; SYSTEM ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332743800001 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1383]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan 430071, Hubei Province, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Hubei Province, Peoples R China 3.Zhejiang Univ, Sch Med, Dept Pharmacol, Hangzhou 310003, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Xin-Hua,Gong, Zhou,Guo, Da-Chuan,et al. A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement[J]. JOURNAL OF BIOMOLECULAR NMR,2014,58(3):149-154. |
| APA | Gu, Xin-Hua,Gong, Zhou,Guo, Da-Chuan,Zhang, Wei-Ping,&Tang, Chun.(2014).A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement.JOURNAL OF BIOMOLECULAR NMR,58(3),149-154. |
| MLA | Gu, Xin-Hua,et al."A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement".JOURNAL OF BIOMOLECULAR NMR 58.3(2014):149-154. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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