Global Metabolomic Responses of Escherichia coli to Heat Stress
文献类型：期刊论文
| 作者 | Ye, Yangfang1,2; Zhang, Limin1; Hao, Fuhua1 ; Zhang, Jingtao1; Wang, Yulan1; Tang, Huiru1
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 11期号:4页码:2559-2566 |
| 关键词 | Escherichia coli heat stress metabolomics nuclear magnetic resonance (NMR) multivariate data analysis |
| 英文摘要 | Microbial metabolomic analysis is essential for understanding responses of microorganisms to heat stress. To understand the comprehensive metabolic responses of Escherichia coli to continuous heat stress, we characterized the metabolomic variations induced by heat stress using NMR spectroscopy in combination with multivariate data analysis. We detected IS amino acids, 10 nucleotides, 9 aliphatic organic acids, 7 amines, glucose and its derivative glucosylglyceric acid, and methanol in the E. coli extracts. Glucosylglyceric acid was reported for the first time in E. coli. We found that heat stress was an important factor influencing the metabolic state and growth process, mainly via suppressing energy associated metabolism, reducing nucleotide biosynthesis, altering amino acid metabolism and promoting osmotic regulation. Moreover, metabolic perturbation was aggravated during heat stress. However, a sign of recovery to control levels was observed after the removal of heat stress. These findings enhanced our understanding of the metabolic responses of E. coli to heat stress and demonstrated the effectiveness of the NMR-based metabolomics approach to study such a complex system. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | TEMPERATURE UP-SHIFT ; GLYCINE-BETAINE ; PROTEIN ; SHOCK ; CATABOLISM ; PUTRESCINE ; SIGMA(32) ; PATHWAY ; REGULON ; GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302388100044 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1543]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Ningbo Univ, Minist Educ, TState Key Lab Magnet Resonance & Atom & Mol Phys, Ningbo 315211, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Yangfang,Zhang, Limin,Hao, Fuhua,et al. Global Metabolomic Responses of Escherichia coli to Heat Stress[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(4):2559-2566. |
| APA | Ye, Yangfang,Zhang, Limin,Hao, Fuhua,Zhang, Jingtao,Wang, Yulan,&Tang, Huiru.(2012).Global Metabolomic Responses of Escherichia coli to Heat Stress.JOURNAL OF PROTEOME RESEARCH,11(4),2559-2566. |
| MLA | Ye, Yangfang,et al."Global Metabolomic Responses of Escherichia coli to Heat Stress".JOURNAL OF PROTEOME RESEARCH 11.4(2012):2559-2566. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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