Systems Responses of Rats to Mequindox Revealed by Metabolic and Transcriptomic Profiling
文献类型：期刊论文
| 作者 | Zhao, Xiu-Ju1; Hao, Fuhua1 ; Huang, Chongyang1; Rantalainen, Mattias2; Lei, Hehua1; Tang, Huiru1; Wang, Yulan1
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2012-09-01 |
| 卷号 | 11期号:9页码:4712-4721 |
| 关键词 | mequindox metabonomics NMR pattern recognition rat transcriptomics |
| 英文摘要 | Mequindox is used as an antibiotic drug in livestock; however, its toxicity remains largely unclear. Previously, we investigated metabolic responses of mice to mequindox exposure. In order to evaluate dependences of animal species in response to mequindox insult, we present the metabolic consequences of mequindox exposure in a rat model, by employing the combination of metabonomics and transcriptomics. Metabolic profiling of urine revealed that metabolic recovery is achieved for rats exposed to a low or moderate dose of mequindox, whereas high levels of mequindox exposure trigger liver dysfunction, causing no such recovery. We found that mequindox exposure causes suppression of the tricarboxylic acid cycle and stimulation of glycolysis, which is in contrast to a mouse model previously investigated. In addition, mequindox dosage induces promotion of beta-oxidation of fatty acids, Fumurute which was confirmed by elevated expressions of acox1, hsd17b2, and cpt1a in liver. Furthermore, altered levels of N-methylnicotinate, 1-methylnicotinamide, and glutathione disu fide highlighted the promotion of vitamin B3 antioxidative cycle in rats exposed to mequindox. Moreover, mequindox exposure altered levels of gut microbiotal related co-metabolites, suggesting a perturbation of the gut microflora of the host. Our work provides a comprehensive view of the toxicological effects of mequindox, which is important in the usage of mequindox in animal and human food safety. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CARNITINE PALMITOYLTRANSFERASE ; METABONOMIC INVESTIGATIONS ; HYDRAZINE TOXICITY ; SERINE DEHYDRATASE ; GUT MICROBIOTA ; MOUSE MODEL ; HUMAN LIVER ; MICE ; EXPRESSION ; SPECTROSCOPY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308389900029 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1681]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Univ Oxford, Dept Stat, Oxford OX1 3TG, England |
| 推荐引用方式 GB/T 7714 | Zhao, Xiu-Ju,Hao, Fuhua,Huang, Chongyang,et al. Systems Responses of Rats to Mequindox Revealed by Metabolic and Transcriptomic Profiling[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(9):4712-4721. |
| APA | Zhao, Xiu-Ju.,Hao, Fuhua.,Huang, Chongyang.,Rantalainen, Mattias.,Lei, Hehua.,...&Wang, Yulan.(2012).Systems Responses of Rats to Mequindox Revealed by Metabolic and Transcriptomic Profiling.JOURNAL OF PROTEOME RESEARCH,11(9),4712-4721. |
| MLA | Zhao, Xiu-Ju,et al."Systems Responses of Rats to Mequindox Revealed by Metabolic and Transcriptomic Profiling".JOURNAL OF PROTEOME RESEARCH 11.9(2012):4712-4721. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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