Streptozotocin-Induced Dynamic Metabonomic Changes in Rat Biofluids
文献类型：期刊论文
| 作者 | Xu, Wenxin1,3; Wu, Junfang1; An, Yanpeng1; Xiao, Chaoni1,2; Hao, Fuhua1 ; Liu, Hongbing1; Wang, Yulan1; Tang, Huiru1
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2012-06-01 |
| 卷号 | 11期号:6页码:3423-3435 |
| 关键词 | metabonomics diabetes unsaturated fatty acids H-1 NMR spectroscopy multivariate data analysis |
| 英文摘要 | Diabetes mellitus is a complex polygenic disease caused by gene-environment interactions with multiple complications, and metabonomic analysis is crucial for pathogenesis, early diagnosis, and timely interventions. Here, we comprehensively analyzed the dynamic metabolic changes in rat urine and plasma, which were induced by the well-known diabetogenic chemical streptozotocin (STZ), using H-1 NMR spectroscopy in conjunction with multivariate data analysis. The results showed that a single intraperitoneal injection of STZ with a moderate dosage (55 mg/kg) induced significant urinary metabonomic changes within 24 h. These changes showed time-dependence and heterogeneity among the treated animals with an animal recovered within 11 days. STZ-induced metabonomic alterations were related to suppression of glycolysis and TCA cycle, promotion of gluconeogenesis and oxidation of amino acids, alterations in metabolisms of basic amino acids associated with diabetic complications, and disruption of lipid metabolism and gut microbiota functions. With diffusion-edited NMR spectral data, we further observed the STZ-induced significant elevation of monounsaturated fatty acids and total unsaturated fatty acids together with reductions in PUFA-to-MUFA ratio in the blood plasma. These findings provided details of the time-dependent metabonomic changes in the progressive development of the STZ-induced diabetes mellitus and showed the possibility of detecting the biochemical changes in the early stage of type 1 diabetic genesis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | DEPENDENT DIABETES-MELLITUS ; MULTIVARIATE STATISTICAL-ANALYSIS ; MAGNETIC-RESONANCE ; H-1-NMR SPECTROSCOPY ; NMR-SPECTROSCOPY ; SYSTEMS BIOLOGY ; GLUCOSE CONTROL ; BETA-CELLS ; INSULIN ; PLASMA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000304682500032 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1683]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.NW Univ Xian, Coll Life Sci, Xian 710069, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Wenxin,Wu, Junfang,An, Yanpeng,et al. Streptozotocin-Induced Dynamic Metabonomic Changes in Rat Biofluids[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(6):3423-3435. |
| APA | Xu, Wenxin.,Wu, Junfang.,An, Yanpeng.,Xiao, Chaoni.,Hao, Fuhua.,...&Tang, Huiru.(2012).Streptozotocin-Induced Dynamic Metabonomic Changes in Rat Biofluids.JOURNAL OF PROTEOME RESEARCH,11(6),3423-3435. |
| MLA | Xu, Wenxin,et al."Streptozotocin-Induced Dynamic Metabonomic Changes in Rat Biofluids".JOURNAL OF PROTEOME RESEARCH 11.6(2012):3423-3435. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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