CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics
文献类型：期刊论文
| 作者 | Li, Jing1,3; Huang, Chongyang2,3; Zheng, Dasheng1; Wang, Yulan2; Yuan, Zhiming1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 11期号:9页码:4654-4661 |
| 关键词 | Lysinibacillus sphaericus catabolite control protein A deficient growth metabolome metabolic networks |
| 英文摘要 | Lysinibacillus sphaericus is a bacterium incapable of metabolizing sugars with the sole exception of N-acetylglucosamine. To unravel the regulatory role of catabolite control protein A (CcpA) in the sugar metabolism of L. sphaericus, a ccpA deficient mutant was constructed by homologous recombination. The mutant showed growth deficiency and a low efficiency of carbon and energy utilization. NMR spectroscopy in combination with multivariate data analysis revealed that the metabolome of L. sphaericus was dominated by 25 metabolites mainly including amino acids, carbohydrate derivatives and organic acids, and that the mutation of the ccpA gene caused significant reduction of leucine, valine, alanine, threonine, glutamate, lysine, D-ornithine, tyrosine, uridine 5'-diphospho-N-acetlyglucosamine formate, fumarate, phenylalanine, aspartate, asparagine, and acetate but elevation of ribose-5-phosphate, and uracil. Furthermore, the networks of CcpA-mediated regulation based on the metabolome were constructed by arrangement of significantly decreasing or increasing metabolites. The network map suggests CcpA regulates and promotes sugar and amino acid metabolism of L. sphaericus. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | GRAM-POSITIVE BACTERIA ; SUBTILIS GNT OPERON ; BACILLUS-SUBTILIS ; CATABOLITE REPRESSION ; PHOSPHOTRANSFERASE SYSTEM ; HPR KINASE/PHOSPHORYLASE ; PROTEOME ANALYSIS ; GENE-EXPRESSION ; SENSOR ENZYME ; CARBON |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308389900023 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1729]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Appl & Environm Microbiol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jing,Huang, Chongyang,Zheng, Dasheng,et al. CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(9):4654-4661. |
| APA | Li, Jing,Huang, Chongyang,Zheng, Dasheng,Wang, Yulan,&Yuan, Zhiming.(2012).CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics.JOURNAL OF PROTEOME RESEARCH,11(9),4654-4661. |
| MLA | Li, Jing,et al."CcpA-Mediated Enhancement of Sugar and Amino Acid Metabolism in Lysinibacillus sphaericus by NMR-Based Metabolomics".JOURNAL OF PROTEOME RESEARCH 11.9(2012):4654-4661. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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