Unraveling the concentration-dependent metabolic response of Pseudomonas sp HF-1 to nicotine stress by H-1 NMR-based metabolomics
文献类型：期刊论文
| 作者 | Ye, Yangfang1; Wang, Xin2; Zhang, Limin3; Lu, Zhenmei2; Yan, Xiaojun1 |
| 刊名 | ECOTOXICOLOGY
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 21期号:5页码:1314-1324 |
| 关键词 | Nicotine Oxidative stress Metabolomics NMR spectroscopy |
| 英文摘要 | Nicotine can cause oxidative damage to organisms; however, some bacteria, for example Pseudomonas sp. HF-1, are resistant to such oxidative stress. In the present study, we analyzed the concentration-dependent metabolic response of Pseudomonas sp. HF-1 to nicotine stress using H-1 NMR spectroscopy coupled with multivariate data analysis. We found that the dominant metabolites in Pseudomonas sp. HF-1 were eight aliphatic organic acids, six amino acids, three sugars and 11 nucleotides. After 18 h of cultivation, 1 g/L nicotine caused significant elevation of sugar (glucose, trehalose and maltose), succinate and nucleic acid metabolites (cytidine, 5'-CMP, guanine 2',3'-cyclic phosphate and adenosine 2',3'-cyclic phosphate), but decrease of glutamate, putrescine, pyrimidine, 2-propanol, diethyl ether and acetamide levels. Similar metabolomic changes were induced by 2 g/L nicotine, except that no significant change in trehalose, 5'-UMP levels and diethyl ether were found. However, 3 g/L nicotine led to a significant elevation in the two sugars (trehalose and maltose) levels and decrease in the levels of glutamate, putrescine, pyrimidine and 2-propanol. Our findings indicated that nicotine resulted in the enhanced nucleotide biosynthesis, decreased glucose catabolism, elevated succinate accumulation, severe disturbance in osmoregulation and complex antioxidant strategy. And a further increase of nicotine level was a critical threshold value that triggered the change of metabolic flow in Pseudomonas sp. HF-1. These findings revealed the comprehensive insights into the metabolic response of nicotine-degrading bacteria to nicotine-induced oxidative toxicity. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Ecology ; Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; MATRICARIA-RECUTITA L. ; PUTIDA STRAIN S16 ; ESCHERICHIA-COLI ; OXIDATIVE STRESS ; H-1-NMR SPECTROSCOPY ; TREHALOSE ; CELLS ; EXPOSURE ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305461500004 |
| 公开日期 | 2015-07-14 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/1599]  |
| 专题 | 武汉物理与数学研究所_数学物理与应用研究部 |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Yangfang,Wang, Xin,Zhang, Limin,et al. Unraveling the concentration-dependent metabolic response of Pseudomonas sp HF-1 to nicotine stress by H-1 NMR-based metabolomics[J]. ECOTOXICOLOGY,2012,21(5):1314-1324. |
| APA | Ye, Yangfang,Wang, Xin,Zhang, Limin,Lu, Zhenmei,&Yan, Xiaojun.(2012).Unraveling the concentration-dependent metabolic response of Pseudomonas sp HF-1 to nicotine stress by H-1 NMR-based metabolomics.ECOTOXICOLOGY,21(5),1314-1324. |
| MLA | Ye, Yangfang,et al."Unraveling the concentration-dependent metabolic response of Pseudomonas sp HF-1 to nicotine stress by H-1 NMR-based metabolomics".ECOTOXICOLOGY 21.5(2012):1314-1324. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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