The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L)
文献类型：期刊论文
| 作者 | Du, Yishuai1; Yi, Mengmeng1,2 ; Xiao, Peng1; Meng, Lingjie1,2; Li, Xian1; Sun, Guoxiang1; Liu, Ying1
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2015-05-01 |
| 卷号 | 44期号:1页码:307-315 |
| 关键词 | Atlantic salmon Aeromonas salmonicida Innate immunity Immune response Early-warning |
| 英文摘要 | Enzyme activities and gene expression of a number of innate immune parameters in the serum, mucus and skin of Atlantic salmon (Salmo salar) were investigated after challenge with a pathogenic strain of Aeromonas salmonicida (A. salmonicida). Fish were injected in the dorsal muscle with either 100 mu l bacterium solution, about 3.05 x 10(7) CPU/ml A. scdmonicida, or 100 mu l 0.9% NaCl (as control group) and tissue samples were collected at days 0, 2, 4 and 6 post-injection. Lysozyme (LSZ) and alkaline phosphatase (AKP) activities in serum, mucus and skin, and LSZ and AKP mRNA expression in skin of the challenged fish were higher than those of the control at most of the experimental time, with significant differences at several time points (P < 0.05), indicating the involvement of LSZ and AKP in the innate immunity of Atlantic salmon to A. salmonicida. Superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities in mucus and skin, along with the SOD, POD and CAT mRNA expression in skin significantly decreased at day 4 and 6, indicating the decreased antioxidant capacity of the challenged fish. Glutamate pyruvate transaminase (GPT) and glutamic oxalacetic transaminase (GOT) activities in serum, mucus and skin of the challenged group were all higher than those of the control after the injection, and at several time points significant differences were found between the two groups, suggesting organs of fish were impaired after the pathogen infection. The changes of the GPT and GOT activities could be used as potential biomarkers for the impairment of physiological functions caused by the pathogen infection. Identified biomarkers of the immune responses will contribute to the early-warning system of the disease. So this study will not only provide a theoretical basis for vaccine development, but also provide basic data for the establishment of early warning systems for diseases caused by A. salmonicida in Atlantic salmon rearing. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353743900037 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/26951]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Yishuai,Yi, Mengmeng,Xiao, Peng,et al. The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(1):307-315. |
| APA | Du, Yishuai.,Yi, Mengmeng.,Xiao, Peng.,Meng, Lingjie.,Li, Xian.,...&Liu, Ying.(2015).The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L).FISH & SHELLFISH IMMUNOLOGY,44(1),307-315. |
| MLA | Du, Yishuai,et al."The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L)".FISH & SHELLFISH IMMUNOLOGY 44.1(2015):307-315. |
入库方式： OAI收割
来源：海洋研究所
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