Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing
文献类型：期刊论文
| 作者 | Sun YN; Chen XD(陈晓东) ; Zhou XG; Zhu JB; Yu YD
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| 刊名 | LAB ON A CHIP
 |
| 出版日期 | 2015 |
| 卷号 | 15期号:11页码:2429-2436 |
| 通讯作者邮箱 | yudeyu@semi.ac.cn |
| ISSN号 | 1473-0197 |
| 产权排序 | [Sun, Yingnan; Zhou, Xiaoguang; Yu, Yude] Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China; [Chen, Xiaodong] Chinese Acad Sci, State Key Lab Nonlinear Mech, Inst Mech, Beijing 100190, Peoples R China; [Zhu, Jinbiao] Chinese Acad Sci, Inst Elect, Beijing 100190, Peoples R China |
| 通讯作者 | Yu, YD (reprint author), Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, POB 912, Beijing 100083, Peoples R China. |
| 中文摘要 | In recent years, inkjet printing, as a new method to fabricate microdroplet microarrays, has been increasingly applied in the field of biochemical diagnostics. To further improve the general applicability of the inkjet printing technology in fabricating biochemical chips, in this work, we introduce a model to describe the multiple injection procedure implemented by the inkjet printing approach, with experimental verification. The multiple injection model demonstrates a new sequential inkjet printing method that generates picoliter-scale multicomponent droplet-in-oil arrays via multistep printing on uniform planar substrates. Based on our previous work on double-inkjet printing, this technique adapts the piezoelectric inkjet printing technology to fabricate an oil droplet array, into which multiple precise injections of secondary droplets with different compositions and volumes can be automatically printed in the required sequence, simultaneously addressing the evaporation issues associated with printing picoliter droplets without external assistance. In this paper, we first describe the theory and characterize the model, which account for the basic principles of sequential inkjet printing, as well as validate the design in terms of multiple injections, droplet fusion, and rapid mixing. The feasibility and effectiveness of the method are also demonstrated in a dual fluorescence assay and a beta-galactosidase enzyme inhibition assay. We believe that applying the sequential inkjet printing methodology in existing inkjet printing devices will enhance their use as universal diagnostic tools as well as accelerate the adoption of inkjet printing in multistep screening experiments. |
| 分类号 | 一类 |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1039/c5lc00356c |
| 语种 | 英语 |
| WOS记录号 | WOS:000354963400010 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/54965]  |
| 专题 | 力学研究所_非线性力学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun YN,Chen XD,Zhou XG,et al. Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing[J]. LAB ON A CHIP,2015,15(11):2429-2436. |
| APA | Sun YN,陈晓东,Zhou XG,Zhu JB,&Yu YD.(2015).Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing.LAB ON A CHIP,15(11),2429-2436. |
| MLA | Sun YN,et al."Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing".LAB ON A CHIP 15.11(2015):2429-2436. |
入库方式： OAI收割
来源：力学研究所
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