Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse
文献类型：期刊论文
| 作者 | Wang SM; Zhang ZY; Lv DY(吕东媛) ; Xu QX
|
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2015-05 |
| 卷号 | 16期号:5页码:9406-9419 |
| 通讯作者邮箱 | sumeiwang@ccmu.edu.cn ; zhenyuzhang@ccmu.edu.cn ; lvdongy@imech.ac.cn ; xuqiuxiang2006@163.com |
| 关键词 | cyclical mechanical stretch estrogen pelvic organ prolapse vaginal fibroblasts |
| ISSN号 | 1422-0067 |
| 产权排序 | [Wang, Sumei; Zhang, Zhenyu; Xu, Qiuxiang] Capital Med Univ, Beijing Chaoyang Hosp, Dept Obstet & Gynecol, Beijing 100020, Peoples R China; [Lu, Dongyuan] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; [Lu, Dongyuan] Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China |
| 通讯作者 | Zhang, ZY (reprint author), Capital Med Univ, Beijing Chaoyang Hosp, Dept Obstet & Gynecol, Beijing 100020, Peoples R China. |
| 中文摘要 | Mechanical load and postmenopausal hypoestrogen are risk factors for pelvic organ prolapse (POP). In this study, we applied a 0.1-Hz uniaxial cyclic mechanical stretching (CS) with 10% elongation and 10(-8) M 17--estradiol to vaginal fibroblasts isolated from postmenopausal women with or without POP to investigate the effects of CS and estrogen on cell morphology and cytoskeletons of normal and POP fibroblasts. Under static culture condition, POP fibroblasts exhibited lower cell circularity and higher relative fluorescence intensities (RFIs) of F-actin, -tubulin and vimentin. When cultured with CS, all fibroblasts grew perpendicular to the force and exhibited a decreased cell projection area, cell circularity and increased cell length/width ratio; normal fibroblasts exhibited increased RFIs of all three types of cytoskeleton, and POP fibroblasts exhibited a decreased RFI of F-actin and no significant differences of -tubulin and vimentin. After being cultured with 17--estradiol and CS, normal fibroblasts no longer exhibited significant changes in the cell projection area and the RFIs of F-actin and -tubulin; POP fibroblasts exhibited no significant changes in cell circularity, length/width ratio and F-actin even with the increased RFIs of -tubulin and vimentin. These findings suggest that POP fibroblasts have greater sensitivity to and lower tolerance for mechanical stretching, and estrogen can improve the prognosis. |
| 分类号 | 二类/Q2 |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.3390/ijms16059406 |
| 语种 | 英语 |
| WOS记录号 | WOS:000356241400019 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/54970]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Wang SM,Zhang ZY,Lv DY,et al. Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2015,16(5):9406-9419. |
| APA | Wang SM,Zhang ZY,吕东媛,&Xu QX.(2015).Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,16(5),9406-9419. |
| MLA | Wang SM,et al."Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 16.5(2015):9406-9419. |
入库方式： OAI收割
来源：力学研究所
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