Identification of the Conformational transition pathway in PIP2 Opening Kir Channels
文献类型：期刊论文
| 作者 | Li JW; Lv SQ(吕守琴); Liu YZ; Pang CL; Chen YF; Zhang SH; Yu H ; Long M(龙勉); Zhang HL; Logothetis DE
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| 刊名 | SCIENTIFIC REPORTS
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| 出版日期 | 2015-06-11 |
| 卷号 | 5页码:11289 |
| 通讯作者邮箱 | zhany@hebut.edu.cn ; hailong_an@hebut.edu.cn |
| ISSN号 | 2045-2322 |
| 产权排序 | [Li, Junwei; Pang, Chunli; Chen, Yafei; Zhang, Suhua; Yu, Hui; Zhan, Yong; An, Hailong] Hebei Univ Technol, Inst Biophys, Sch Sci, Key Lab Mol Biophys, Tianjin 300401, Hebei Province, Peoples R China; [Lu, Shouqin; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; [Lu, Shouqin; Long, Mian] Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China; [Liu, Yuzhi] Shijiazhuang Tiedao Univ, Sch Elect & Elect Engn, Shijiazhuang 050043, Peoples R China; [Zhang, Hailin] Hebei Med Univ, Dept Pharmacol, Key Lab Pharmacol & Toxicol New Drug, Minist Educ,Key Lab Neural & Vasc Biol, Shijiazhuang 050017, Hebei Province, Peoples R China; [Logothetis, Diomedes E.] Virginia Commonwealth Univ, Sch Med, Dept Physiol & Biophys, Richmond, VA 23298 USA |
| 通讯作者 | An, HL (reprint author), Hebei Univ Technol, Inst Biophys, Sch Sci, Key Lab Mol Biophys, Tianjin 300401, Hebei Province, Peoples R China. |
| 中文摘要 | The gating of Kir channels depends critically on phosphatidylinositol 4,5-bisphosphate (PIP2), but the detailed mechanism by which PIP2 regulates Kir channels remains obscure. Here, we performed a series of Targeted molecular dynamics simulations on the full-length Kir2.1 channel and, for the first time, were able to achieve the transition from the closed to the open state. Our data show that with the upward motion of the cytoplasmic domain (CTD) the structure of the C-Linker changes from a loop to a helix. The twisting of the C-linker triggers the rotation of the CTD, which induces a small downward movement of the CTD and an upward motion of the slide helix toward the membrane that pulls the inner helix gate open. At the same time, the rotation of the CTD breaks the interaction between the CD- and G-loops thus releasing the G-loop. The G-loop then bounces away from the CD-loop, which leads to the opening of the G-loop gate and the full opening of the pore. We identified a series of interaction networks, between the N-terminus, CD loop, C linker and G loop one by one, which exquisitely regulates the global conformational changes during the opening of Kir channels by PIP2. |
| 分类号 | 一类 |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1038/srep11289 |
| 语种 | 英语 |
| WOS记录号 | WOS:000356099300001 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/54972]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Li JW,Lv SQ,Liu YZ,et al. Identification of the Conformational transition pathway in PIP2 Opening Kir Channels[J]. SCIENTIFIC REPORTS,2015,5:11289. |
| APA | Li JW.,Lv SQ.,Liu YZ.,Pang CL.,Chen YF.,...&An HL.(2015).Identification of the Conformational transition pathway in PIP2 Opening Kir Channels.SCIENTIFIC REPORTS,5,11289. |
| MLA | Li JW,et al."Identification of the Conformational transition pathway in PIP2 Opening Kir Channels".SCIENTIFIC REPORTS 5(2015):11289. |
入库方式： OAI收割
来源：力学研究所
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