A competitive low-affinity binding model for determining the mutual and specific sites of two ligands on protein
文献类型：期刊论文
| 作者 | Bai, GY; Cui, YF; Yang, YH; Ye, CH; Liu, ML |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2005-07-15 |
| 卷号 | 38期号:4页码:588-593 |
| 关键词 | competitive binding mutual binding sites NMR relaxation rate human serum albumin tolmetin salicylic acid |
| 英文摘要 | A competitive low-affinity binding model was proposed for determining the number of mutual (overlapped) and specific binding sites of two ligands (A, B) on a protein (P). To use the model, one needs to carry out a titration experiment by adding either ligand A or B into a three-component system (A-B-P), and to monitor the spectroscopic parameter changes. Fitting the titration curve to the proposed model, one can get the mutual and specific binding sites of the two ligands on the protein. The model was examined by using human serum albumin (HSA) as a receptor and tolmetin (TOL) and salicylic acid (SAL) as ligands. Proton longitudinal relaxation rates (R-1) were measured on a 500-MHz NMR spectrometer during the titration and used to derive the mutual binding sites. It was found that among the binding sites of 32 +/- 4 for SAL and 28 +/- 2 for TOL on HSA, there were 17 +/- 5 mutual sites for the two ligands. This result indicates that, although HSA has large binding capacities for most ligands, there are still a reasonable amount of the low-affinity binding sites that are structure selective. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| 类目[WOS] | Chemistry, Analytical ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Chemistry ; Pharmacology & Pharmacy |
| 关键词[WOS] | HUMAN-SERUM-ALBUMIN ; NMR DIFFUSION MEASUREMENTS ; RELAXATION ; DRUGS ; ENANTIOMERS ; IBUPROFEN ; TOLMETIN ; PLASMA ; PROBE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230718000002 |
| 公开日期 | 2015-07-28 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/4510]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Cent China Normal Univ, Dept Chem, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, GY,Cui, YF,Yang, YH,et al. A competitive low-affinity binding model for determining the mutual and specific sites of two ligands on protein[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,38(4):588-593. |
| APA | Bai, GY,Cui, YF,Yang, YH,Ye, CH,&Liu, ML.(2005).A competitive low-affinity binding model for determining the mutual and specific sites of two ligands on protein.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,38(4),588-593. |
| MLA | Bai, GY,et al."A competitive low-affinity binding model for determining the mutual and specific sites of two ligands on protein".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 38.4(2005):588-593. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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