Multivariate analysis of integrated and full-resolution H-1-NMR spectral data from complex pharmaceutical preparations: St. John's wort
文献类型：期刊论文
| 作者 | Rasmussen, B; Cloarec, O; Tang, HR; Staerk, D; Jaroszewski, JW |
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 72期号:6页码:556-563 |
| 关键词 | St. John's wort NMR spectroscopy pattern recognition principal component analysis data reduction quality control |
| 英文摘要 | Commercial herbal preparations are typically very complex mixtures and the relationship between content of various constituents and pharmacological action of the formulation is usually unclear. Such formulations are nevertheless standardized using a single marker constituent or a group of closely related constituents, which provides no information about other abundant constituents present in the extract. In this study, principal component analysis of 600 MHz H-1-NMR spectra of extracts of commercial formulations of St. John's wort (Hypericum perforatum), acquired in methanol-d(4) and DMSO-d(6), was shown to be able to discriminate between various preparations according to their global composition, including differentiation between various batches from the same supplier, while no clustering into classes of tablets and capsules was observed. This suggests that the plant extract variability rather than the manufacturing process accounts for the data clustering. Major variations in the content of flavonoids, recently linked to the antidepressant activity of St. John's wort extracts, were detected. Use of two NMR solvents provided complementary data sets, allowing assessment of various aspects of sample composition from separate PCA models. Both integrated (about 200 variables) and full-resolution NMR data (about 30000 variables) have been used. The latter approach, applied for the first time in analysis of a herbal preparation, provided via loading plots more precise information about constituents responsible for data clustering, and may be generally preferable for PCA analysis of NMR data of plant extracts and herbal medicines. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Plant Sciences ; Pharmacology & Pharmacy |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; PRINCIPAL COMPONENT ANALYSIS ; FORCED SWIMMING TEST ; ANTIDEPRESSANT ACTIVITY ; CLASSIFICATION ; EXTRACTS ; METABONOMICS ; FLAVONOIDS ; DEPRESSION ; PLANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238018100014 |
| 公开日期 | 2015-07-28 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/4641]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Danish Univ Pharmaceut Sci, Dept Med Chem, DK-2100 Copenhagen, Denmark 2.Univ London Imperial Coll Sci & Technol, Fac Med, Div Biomed Sci, Sect Biol Chem, London, England 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Mol & Atom Phys, Wuhan Inst Phys & Math, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Rasmussen, B,Cloarec, O,Tang, HR,et al. Multivariate analysis of integrated and full-resolution H-1-NMR spectral data from complex pharmaceutical preparations: St. John's wort[J]. PLANTA MEDICA,2006,72(6):556-563. |
| APA | Rasmussen, B,Cloarec, O,Tang, HR,Staerk, D,&Jaroszewski, JW.(2006).Multivariate analysis of integrated and full-resolution H-1-NMR spectral data from complex pharmaceutical preparations: St. John's wort.PLANTA MEDICA,72(6),556-563. |
| MLA | Rasmussen, B,et al."Multivariate analysis of integrated and full-resolution H-1-NMR spectral data from complex pharmaceutical preparations: St. John's wort".PLANTA MEDICA 72.6(2006):556-563. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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