High-resolution magic-angle spinning C-13 spectroscopy of brain tissue at natural abundance
文献类型：期刊论文
| 作者 | Yang, YX; Chen, L; Gao, HC; Zeng, DL; Yue, Y; Liu, ML; Lei, H; Deng, F; Ye, CH |
| 刊名 | MAGNETIC RESONANCE IN CHEMISTRY
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 44期号:3页码:263-268 |
| 关键词 | C-13 NMR spectroscopy natural abundance magic-angle spinning DEPT NOE-CPMG brain |
| 英文摘要 | High-resolution magic-angle spinning (MAS) H-1 and C-13 magnetic resonance spectroscopy (MRS) has recently been applied to study the metabolism in intact biological tissue samples. Because of the low natural abundance and the low gyromagnetic ratio of the C-13 nuclei, signal enhancement techniques such as cross-polarization(CP) and distortionless enhancement by polarization transfer (DEPT) are often employed in MAS C-13 MRS to improve the detection sensitivity. In this study, several sensitivity enhancement techniques commonly used in liquid- and solid-state NMR, including CP, DEPT and nuclear Overhauser enhancement (NOE), were combined with MAS to acquire high-resolution C-13 spectra on intact rat brain tissue at natural abundance, and were compared for their performances. The results showed that different signal enhancement techniques are sensitive to different classes of molecules/metabolites, depending on their molecular weights and mobility. DEPT was found to enhance the signals of low-molecular weight metabolites exclusively, while the signals of lipids, which often are associated with membranes and have relatively lower mobility, were highly sensitive to CP enhancement. Copyright (c) 2006 John Wiley & Sons, Ltd. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Chemistry, Physical ; Spectroscopy |
| 研究领域[WOS] | Chemistry ; Spectroscopy |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; IN-VIVO ; NMR-SPECTROSCOPY ; H-1-NMR SPECTROSCOPY ; MRS ; POLARIZATION ; MYELIN ; TUMORS ; VITRO ; H-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236013800009 |
| 公开日期 | 2015-07-28 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/4682]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, YX,Chen, L,Gao, HC,et al. High-resolution magic-angle spinning C-13 spectroscopy of brain tissue at natural abundance[J]. MAGNETIC RESONANCE IN CHEMISTRY,2006,44(3):263-268. |
| APA | Yang, YX.,Chen, L.,Gao, HC.,Zeng, DL.,Yue, Y.,...&Ye, CH.(2006).High-resolution magic-angle spinning C-13 spectroscopy of brain tissue at natural abundance.MAGNETIC RESONANCE IN CHEMISTRY,44(3),263-268. |
| MLA | Yang, YX,et al."High-resolution magic-angle spinning C-13 spectroscopy of brain tissue at natural abundance".MAGNETIC RESONANCE IN CHEMISTRY 44.3(2006):263-268. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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