Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy
文献类型：期刊论文
| 作者 | Ye, Yansheng1,2; Liu, Xiaoli1; Xu, Guohua1; Liu, Maili1; Li, Conggang1 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2015-04-27 |
| 卷号 | 54期号:18页码:5328-5330 |
| 关键词 | calmodulin fluorine NMR spectroscopy protein conformation signal transduction |
| 英文摘要 | calmodulin (CaM) is essential to a variety of signal transduction pathways. Whether the transition in living cells is similar to that observed in buffer is not known. Here, we report the direct observation by F-19 NMR spectroscopy of the transition of the Ca2+-free and -bound forms in Xenopus laevis oocytes at different Ca2+ levels. We find that the Ca2+-bound CaM population increased greatly upon binding the target protein myosin light-chain kinase (MLCK) at the same Ca2+ level. Paramagnetic NMR spectroscopy was also exploited for the first time to obtain long-range structural constraints in cells. Our study shows that F-19 NMR spectroscopy can be used to obtain long-range structural constraints in living eukaryotic cells and paves the way for quantification of protein binding constants. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; ESCHERICHIA-COLI ; PROTEIN INTERACTIONS ; IN-VIVO ; CELLS ; REVEALS ; KINASE ; CA2+ ; WEAK |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000354191600007 |
| 公开日期 | 2015-07-28 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/4740]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Yansheng,Liu, Xiaoli,Xu, Guohua,et al. Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(18):5328-5330. |
| APA | Ye, Yansheng,Liu, Xiaoli,Xu, Guohua,Liu, Maili,&Li, Conggang.(2015).Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(18),5328-5330. |
| MLA | Ye, Yansheng,et al."Direct Observation of Ca2+-Induced Calmodulin Conformational Transitions in Intact Xenopus laevis Oocytes by F-19 NMR Spectroscopy".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.18(2015):5328-5330. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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