Labeling Strategy and Signal Broadening Mechanism of Protein NMR Spectroscopy in Xenopus laevis Oocytes
文献类型：期刊论文
| 作者 | Ye, Yansheng1,2; Liu, Xiaoli1; Chen, Yanhua1,2; Xu, Guohua1; Wu, Qiong1; Zhang, Zeting1; Yao, Chendie1,2; Liu, Maili1; Li, Conggang1 |
| 刊名 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| 出版日期 | 2015-06-08 |
| 卷号 | 21期号:24页码:8686-8690 |
| 关键词 | in-cell NMR spectroscopy NMR spectroscopy proteins Xenopus laevis oocytes |
| 英文摘要 | We used Xenopus laevis oocytes, a paradigm for a variety of biological studies, as a eukaryotic model system for in-cell protein NMR spectroscopy. The small globular protein GB1 was one of the first studied in Xenopus oocytes, but there have been few reports since then of high-resolution spectra in oocytes. The scarcity of data is at least partly due to the lack of good labeling strategies and the paucity of information on resonance broadening mechanisms. Here, we systematically evaluate isotope enrichment and labeling methods in oocytes injected with five different proteins with molecular masses of 6 to 54kDa. F-19 labeling is more promising than N-15, C-13, and H-2 enrichment. We also used (FNMR)-F-19 spectroscopy to quantify the contribution of viscosity, weak interactions, and sample inhomogeneity to resonance broadening in cells. We found that the viscosity in oocytes is only about 1.2times that of water, and that inhomogeneous broadening is a major factor in determining line width in these cells. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | IN-CELL NMR ; NUCLEAR-MAGNETIC-RESONANCE ; F-19 NMR ; ESCHERICHIA-COLI ; LIVING CELLS ; ROTATIONAL MOBILITY ; DYNAMICS ; VIVO ; WEAK |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355287000003 |
| 公开日期 | 2015-07-28 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/4746]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Ctr Magnet Resonance Dept,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Yansheng,Liu, Xiaoli,Chen, Yanhua,et al. Labeling Strategy and Signal Broadening Mechanism of Protein NMR Spectroscopy in Xenopus laevis Oocytes[J]. CHEMISTRY-A EUROPEAN JOURNAL,2015,21(24):8686-8690. |
| APA | Ye, Yansheng.,Liu, Xiaoli.,Chen, Yanhua.,Xu, Guohua.,Wu, Qiong.,...&Li, Conggang.(2015).Labeling Strategy and Signal Broadening Mechanism of Protein NMR Spectroscopy in Xenopus laevis Oocytes.CHEMISTRY-A EUROPEAN JOURNAL,21(24),8686-8690. |
| MLA | Ye, Yansheng,et al."Labeling Strategy and Signal Broadening Mechanism of Protein NMR Spectroscopy in Xenopus laevis Oocytes".CHEMISTRY-A EUROPEAN JOURNAL 21.24(2015):8686-8690. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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