Heterodimerization of integrin Mac-1 subunits studied by single-molecule imaging
文献类型：期刊论文
| 作者 | Fu Guo ; Wang Chen ; Liu Li ; Wang Gui-ying ; Chen Yi-zhang ; Xu ZZ(徐至展) |
| 刊名 | biochem. biophys. res. commun.
 |
| 出版日期 | 2008 |
| 卷号 | 368期号:4页码:882 |
| 关键词 | Mac-1 CD11b single-molecule dimerization imaging integrins diffusion YFP microscopy CHO |
| ISSN号 | 0006-291x |
| 中文摘要 | heterodimerization of integrin mac-1 (alpha(m) beta(2)) subunits plays important role on regulating leukocytes adhesion to extracellular matrix or endothelial cells. here, using total internal reflection microscopy, we investigated the heterodimerization of integrin mac-1 subunits at the single-molecule level in live cells. individual alpha(m) subunit fused to the enhanced yellow fluorescent protein (eyfp) was imaged at the basal plasma membrane of live chinese hamster ovary (cho) cells. through analysis of mean square displacement (msd), diffusion coefficient, the size of restricted domain and fraction of molecules undergoing restricted diffusion, we found that as compared with the diffusion in the absence of beta(2) subunit, the diffusion of single-molecule of alpha(m)-yfp was suppressed significantly in the presence of beta(2) subunit. thus, based on the oligomerization-induced trapping model, we suggested that in the presence of beta(2) subunit, the am subunit may form heterodimer with it. (c) 2008 elsevier inc. all rights reserved. |
| 学科主题 | 激光技术;激光物理与基本理论 |
| 语种 | 英语 |
| WOS记录号 | WOS:000254106700008 |
| 公开日期 | 2009-09-18 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/532]  |
| 专题 | 上海光学精密机械研究所_强场激光物理国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Fu Guo,Wang Chen,Liu Li,et al. Heterodimerization of integrin Mac-1 subunits studied by single-molecule imaging[J]. biochem. biophys. res. commun.,2008,368(4):882, 886. |
| APA | Fu Guo,Wang Chen,Liu Li,Wang Gui-ying,Chen Yi-zhang,&徐至展.(2008).Heterodimerization of integrin Mac-1 subunits studied by single-molecule imaging.biochem. biophys. res. commun.,368(4),882. |
| MLA | Fu Guo,et al."Heterodimerization of integrin Mac-1 subunits studied by single-molecule imaging".biochem. biophys. res. commun. 368.4(2008):882. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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