Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China
文献类型：期刊论文
| 作者 | Li, Aifeng2; Tian, Zhijia2; Li, Jing2 ; Yu, Rencheng3; Banack, Sandra Anne1; Wang, Zhenyu2
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| 刊名 | TOXICON
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| 出版日期 | 2010-05-01 |
| 卷号 | 55期号:5页码:947-953 |
| 关键词 | beta-N-methylamino-L-alanine (BMAA) Neurotoxin Cyanobacteria Nostoc sp. Microcystis aeruginosa Liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) |
| 英文摘要 | The cyanobacterial neurotoxin, beta-N-methylamino-L-alanine (BMAA), has been suggested as an important environmental factor for neurodegenerative disease such as amyotrophic lateral sclerosis- Parkinsonism dementia complex (ALS/PDC) in Guam. BMAA was detected within the majority of cyanobacterial isolates surveyed in both free and symbiotic cyanobacteria, living in freshwater as well as marine environments. In this study, we report two methods using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) each coupled with a different type of hydrophilic interaction liquid chromatography (HILIC) column to detect BMAA. A third method using AQC-derivatized BMAA was also used for comparison. Axenic cultures of Microcystis aeruginosa and Nostoc sp. isolated from Chinese freshwater were analyzed for both free and protein-bound BMAA at the exponential growth stage. Cultures of two strains of M. aeruginosa collected at four growth stages were also analyzed for the presence of BMAA. BMAA was detected in the Nostoc sp. at very low concentrations (<0.07 pmoles on column) only when precolumn AQC derivatization was used. No BMAA was detected in the Chinese derived axenic cultures of Microcystis; detection limits for the LC-ESI-MS and LC-ESI-MS/MS without precolumn derivatization were 10 ng and 2 pg BMAA on column, respectively. We suggest that cyanobacteria grown under some culture conditions may be relatively free of BMAA. (C) 2009 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276436400006 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32707]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Inst Ethnomed, Jackson, WY 83001 USA 2.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China 3.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Aifeng,Tian, Zhijia,Li, Jing,et al. Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China[J]. TOXICON,2010,55(5):947-953. |
| APA | Li, Aifeng,Tian, Zhijia,Li, Jing,Yu, Rencheng,Banack, Sandra Anne,&Wang, Zhenyu.(2010).Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China.TOXICON,55(5),947-953. |
| MLA | Li, Aifeng,et al."Detection of the neurotoxin BMAA within cyanobacteria isolated from freshwater in China".TOXICON 55.5(2010):947-953. |
入库方式： OAI收割
来源：海洋研究所
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