Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity
文献类型：期刊论文
| 作者 | Zheng, Y. L.3; Lu, X. L.1; Lin, J.1; Chen, H. M.1; Yan, X. J.1; Wang, F.2 ; Xu, W. F.2
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| 刊名 | BIOMEDICINE & PHARMACOTHERAPY
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| 出版日期 | 2010-10-01 |
| 卷号 | 64期号:8页码:527-533 |
| 关键词 | Fascaplysin Anti-angiogenesis HUVEC G1 cell cycle arrest Apoptosis |
| 英文摘要 | Novel anti-angiogenesis activity of fascaplysin via VEGF blockage was recently revealed by our previous study in addition to the reported cyclin-dependent kinase 4 (CDK4) selective inhibition. To uncover more details of this pharmacologically prospective property, this study further investigated whether fascaplysin had direct anti-proliferation effects on human umbilical vein endothelial cells (HUVEC), which might be contributing to anti-angiogenesis. The results showed that Cl cell cycle arrest was induced by 2.6 mu M fascaplysin in a time-dependent manner, and exhibited more sensitive than hepatocarcinoma cells BeL-7402 and Hela cells. Approximately 56.09 +/- 2.63% of the cells were arrested at the Cl phase after 24 h, and 64.94 +/- 2.07% after 36 h, comparing to the 22.82 +/- 1.2% in methanol treated cells. Apoptosis of HUVEC cells was induced by 1.3 mu M fascaplysin and indicated by the sub-G1, Hoechst staining, terminal deoxynucleotidyl transferase dUTP-mediated nicked end labeling (TUNEL) assay, and annexin-V and propidium (PI) label. This apoptosis response was further confirmed by the detection of active caspase-3 and by western blotting using antibodies against Bax, Bcl-2, procaspase-8, and Bid, indicating that apoptosis in HUVEC cells may involve a mitochondria pathway, by the demonstration of an increase in the Bax/Bcl-2 ratio. Together, our results suggest that the anti-angiogenesis activity of fascaplysin is through the direct effects of cell cycle arrest and apoptosis on HUVEC. (C) 2009 Elsevier Masson SAS. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283571100004 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32730]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Ningbo Univ, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo, Zhejiang, Peoples R China 2.Lihuili Hosp, Ningbo Med Ctr, Dept Clin Lab, Ningbo 315041, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Y. L.,Lu, X. L.,Lin, J.,et al. Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity[J]. BIOMEDICINE & PHARMACOTHERAPY,2010,64(8):527-533. |
| APA | Zheng, Y. L..,Lu, X. L..,Lin, J..,Chen, H. M..,Yan, X. J..,...&Xu, W. F..(2010).Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity.BIOMEDICINE & PHARMACOTHERAPY,64(8),527-533. |
| MLA | Zheng, Y. L.,et al."Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity".BIOMEDICINE & PHARMACOTHERAPY 64.8(2010):527-533. |
入库方式： OAI收割
来源：海洋研究所
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