Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino
文献类型：期刊论文
| 作者 | Qi, Haigang; Liu, Xiao; Wu, Fucun; Zhang, Guofan |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 37期号:8页码:3779-3784 |
| 关键词 | SNPs Pacific abalone Haliotis discus hannai Ino Direct sequencing AS-PCR |
| 英文摘要 | Useful and novel DNA markers are needed for aquaculture genetics and breeding. In this study, we report the discovery and development of gene-targeted single nucleotide polymorphisms (SNPs) for genomic mapping in the Pacific abalone Haliotis discus hannai Ino. Single EST or EST-contigs from 66 genes that had positive BLASTx matches (E-value a parts per thousand currency sign 1e-8) were used for polymerase chain reaction (PCR) amplification. PCR products from the two parents of one mapping family were directly sequenced, and 83 SNP loci were found from 17 genes. Allele-specific PCR (AS-PCR) was developed and optimized for genotyping of 11 SNP loci in 120 progeny of the mapping family. Nine of the loci conformed to the expected Mendelian ratio of 1:1 based on the chi(2) test (P > 0.05) and could potentially be used for linkage map construction. Our data also indicate that the sequencing of two parents may be a practical strategy for the discovery of informative SNPs for linkage mapping in a particular mapping population. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283577400016 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32760]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Haigang,Liu, Xiao,Wu, Fucun,et al. Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino[J]. MOLECULAR BIOLOGY REPORTS,2010,37(8):3779-3784. |
| APA | Qi, Haigang,Liu, Xiao,Wu, Fucun,&Zhang, Guofan.(2010).Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino.MOLECULAR BIOLOGY REPORTS,37(8),3779-3784. |
| MLA | Qi, Haigang,et al."Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino".MOLECULAR BIOLOGY REPORTS 37.8(2010):3779-3784. |
入库方式： OAI收割
来源：海洋研究所
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