Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye
文献类型：期刊论文
| 作者 | Feng, Jinhua1,2; Yu, Huahua1 ; Li, Cuiping1,2; Xing, Ronge1; Liu, Song1; Wang, Lin1,2; Cai, Shengbao1,2; Li, Pengcheng1
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| 刊名 | TOXICON
 |
| 出版日期 | 2010 |
| 卷号 | 55期号:1页码:118-125 |
| 关键词 | Jellyfish Cyanea nozakii Kishinouye Nematocyst Lethality Isolation |
| 英文摘要 | Cyanea nozakii Kishinouye, a jellyfish widely distributed in coastal areas of China, has garnered attention because of its stinging capacity and the resulting public health hazard. We used a recently developed technique to extract jellyfish venom from nematocysts; the present study investigates the lethality of C. nozakii venom. The nematocyst contents were extremely toxic to the grass carp, Ctenopharyngodon idellus, producing typical neurotoxin toxicity. The ID50 was about 0.6 mu g protein/g fish. Toxin samples were stable when kept at -80 degrees C, but after 48 h, an 80% decline in lethality occurred at -20 degrees C. Poor stability of the venom was observed within the range of 65-80 degrees C and at pH 3.5. The venom was hydrolyzed by a proteolytic enzyme, trypsin. Fractionation of the venom yielded two protein bands with molecular weights of 60 kDa and 50 kDa. Our results provide the first evidence that C. nozakii produces lethal toxins. These characteristics highlight the need for the isolation and molecular characterization of new active toxins in C nozakii. (C) 2009 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273921500015 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32769]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Jinhua,Yu, Huahua,Li, Cuiping,et al. Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye[J]. TOXICON,2010,55(1):118-125. |
| APA | Feng, Jinhua.,Yu, Huahua.,Li, Cuiping.,Xing, Ronge.,Liu, Song.,...&Li, Pengcheng.(2010).Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye.TOXICON,55(1),118-125. |
| MLA | Feng, Jinhua,et al."Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye".TOXICON 55.1(2010):118-125. |
入库方式： OAI收割
来源：海洋研究所
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