Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas
文献类型：期刊论文
| 作者 | Wang, Xiaotong; Li, Li; Xu, Fei; Zhang, Guofan |
| 刊名 | ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
 |
| 出版日期 | 2011 |
| 卷号 | 63 |
| 关键词 | oyster live sampling genomic DNA MgSO4 anesthesia |
| 英文摘要 | As opposed to other animals, live bivalve shellfish cannot be sampled because of the high mortality associated with opening closed shells. In this paper, we present a new anesthesia method using an MgSO4 seawater solution for sampling live bivalve shellfish. In our experiment, genomic DNA was successfully extracted from mantle tissues of sampled Pacific oyster (Crassostrea gigas) and the DNA was used as a template for polymerase chain reaction (PCR), which indicated that 3 mm(3) of mantle tissue was enough for DNA extraction and subsequent molecular biology testing. The survival rate after the in vivo sampling was high (83.3%), indicating that the sampling method caused only slight harm to the oysters. Thus, MgSO4 anesthesia is a practical method for in vivo sampling of oysters and probably could be applied to other bivalve shellfish species as well, significantly promoting the application of molecular marker-assisted selection in bivalve shellfish breeding and the study of tissue injury and repair mechanisms in bivalve shellfish. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298952800001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32932]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaotong,Li, Li,Xu, Fei,et al. Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas[J]. ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,2011,63. |
| APA | Wang, Xiaotong,Li, Li,Xu, Fei,&Zhang, Guofan.(2011).Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas.ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,63. |
| MLA | Wang, Xiaotong,et al."Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas".ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH 63(2011). |
入库方式： OAI收割
来源：海洋研究所
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