An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea
文献类型：期刊论文
| 作者 | Gao, ZQ; Wang, GC; Tseng, CK |
| 刊名 | INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 42期号:1页码:41-47 |
| 关键词 | Porphyra yezoensis Udea thylakoid membranes PSII ultracentrifugation differential speeds centrifugation SDS-PAGE absorption spectra fluorescence spectra |
| 英文摘要 | An improved method for isolation and characterization of photosystem (PS)II particles from thylakoid membranes of gametophytes. of a marine alga Porphyra yezoensis Udea is reported. Thylakoid membranes were isolated using ultracentrifugation and differential speeds centrifugation and were further purified by the first sucrose density gradient centrifugation (SDGC). PSII particles with high 2, 6-dichloroindophenol (DCIP) photo-reduction activity were isolated by the second SDGC from the thylakoid membranes. Absorption and fluorescence spectra of the thylakoid membranes and PSII particles were recorded and their polypeptides composition was studied. Thylakoid membranes obtained by the above two methods showed similar spectral properties and polypeptides composition. PSII particles, in addition to common extrinsic proteins found in PSII of other plants, contained cyt c-550, a 20 kDa protein, along with two new proteins (14 kDa and 16 kDa). |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227505100006 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32525]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, ZQ,Wang, GC,Tseng, CK. An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea[J]. INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,2005,42(1):41-47. |
| APA | Gao, ZQ,Wang, GC,&Tseng, CK.(2005).An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea.INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,42(1),41-47. |
| MLA | Gao, ZQ,et al."An improved method for isolation of photosystem II from marine alga Porphyra yezoensis Udea".INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS 42.1(2005):41-47. |
入库方式： OAI收割
来源：海洋研究所
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