Genetic transformation of marine Actinomycete sp isolate M048 and expression of a recombinant plasmid carrying the apc gene
文献类型：期刊论文
| 作者 | Hou Yanhua; Li Fuchao; Qin Song; Wang Quanfu |
| 刊名 | ACTA OCEANOLOGICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 25期号:6页码:145-152 |
| 关键词 | allophycocyanin protoplast intergeneric conjugantion exconjugant marine Actinomycetes |
| 英文摘要 | Optimal conditions for protoplasts formation of marine Actinomycete sp. isolate M048 were described, dense and disperse mycelia were cultured in SGGP medium, 0.5% glycine, lysozyme exposure (2 mg/cm(3), 37 degrees C, 40 min), and the concentration of sucrose in protoplast buffer was 0.4 mol/dm(3) for keeping the balance of osmotic pressure. Using PEG-mediated protoplasts transformation, the transformation frequency was 89 transformants per microgramme of pIJ702. Meanwhile, an effective transformation procedure was established based on intergeneric conjugation from E. coli ET12567 ( pUZ8002) using shuttle vectors pPM801, pPM803 and a phi C31-derived integration vector pIJ8600 containing oriT and attP fragments. Transformation frequencies were 5.30 x 10(-4) +/- 0.26 x 10(-4), 8.92 x 10(-4) +/- 0.19 x 10(-4) and 6.38 x 10(-5) +/- 0.41 x 10(-5), respectively. Further, the heterologous expression of the allophycocyanin gene (apc) in the strain M048 was used to demonstrate this transformation system. SDS - PAGE and Western blot analysis confirmed the expression of recombinant APC (rAPC). |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243201300015 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32532]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Harbin Inst Technol, Sch Ocean, Weihai 264209, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou Yanhua,Li Fuchao,Qin Song,et al. Genetic transformation of marine Actinomycete sp isolate M048 and expression of a recombinant plasmid carrying the apc gene[J]. ACTA OCEANOLOGICA SINICA,2006,25(6):145-152. |
| APA | Hou Yanhua,Li Fuchao,Qin Song,&Wang Quanfu.(2006).Genetic transformation of marine Actinomycete sp isolate M048 and expression of a recombinant plasmid carrying the apc gene.ACTA OCEANOLOGICA SINICA,25(6),145-152. |
| MLA | Hou Yanhua,et al."Genetic transformation of marine Actinomycete sp isolate M048 and expression of a recombinant plasmid carrying the apc gene".ACTA OCEANOLOGICA SINICA 25.6(2006):145-152. |
入库方式： OAI收割
来源：海洋研究所
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