Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus)
文献类型：期刊论文
| 作者 | Qiu, LH; Song, LS; Wu, LT; Cai, ZH; Jiang, SG |
| 刊名 | ACTA OCEANOLOGICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 25期号:1页码:127-136 |
| 关键词 | Japanese sea perch interleukin-1 beta gene cloning cDNA mRNA expression |
| 英文摘要 | The technology of homology cloning and anchored PCR was used to clone the IL-1 beta gene from the Japanese sea perch (Lateolabrax japonicus). The full-length cDNA of sea perch IL-1 beta was 1 310 bp, including a 5' untranslated region (UTR) of 136 bp, a 3' UTR of 430 bp, and an ORF of 774 bp encoding a polypeptide of 258 amino acids with an estimated molecular mass of 29.31 kDa. The searches for nucleotides and protein sequence similarities with the BLAST analysis indicated that the deduced amino acid sequence of sea perch IL-1 beta was homological to the IL-1 beta in other fish species and even the mammalian. Conserved signature sequences of the IL-1 beta gene family were found in the sea perch IL-1 beta deduced amino acid sequence. Temporal expressions of the IL-1 beta gene in LPS or iridovirus challenged group and in control group were measured by the semi-quantitative RT-PCR. The mRNA transcripts of IL-1 beta could be detected in head-kidney, spleen, liver, gill and heart of the healthy individuals, and the expression level of IL-1 beta in head-kidney, spleen and gill was higher than that in liver and heart, but it was hard to be detected in the brain. After being stimulated by the LPS or iridovirus, the IL-1 beta expression in most of examined tissues was up-regulated, and also could be detected in the brain. These results indicated that the expression of sea perch IL-1 beta was constitutive and could be up-regulated by immune effector stimulation. Therefore the sea perch IL-1 beta could play a critical role in the host-pathogen interaction. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235984300013 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32558]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, LH,Song, LS,Wu, LT,et al. Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus)[J]. ACTA OCEANOLOGICA SINICA,2006,25(1):127-136. |
| APA | Qiu, LH,Song, LS,Wu, LT,Cai, ZH,&Jiang, SG.(2006).Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus).ACTA OCEANOLOGICA SINICA,25(1),127-136. |
| MLA | Qiu, LH,et al."Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus)".ACTA OCEANOLOGICA SINICA 25.1(2006):127-136. |
入库方式： OAI收割
来源：海洋研究所
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