Microbial diversity in polluted harbor sediments II: Sulfate-reducing bacterial community assessment using terminal restriction fragment length polymorphism and clone library of dsrAB gene
文献类型：期刊论文
| 作者 | Zhang, Wen1,2; Song, Lin-Sheng3; Ki, Jang-Seu1,2; Lau, Chun-Kwan1,2; Li, Xiang-Dong4; Qian, Pei-Yuan1,2 |
| 刊名 | ESTUARINE COASTAL AND SHELF SCIENCE
 |
| 出版日期 | 2008-02-10 |
| 卷号 | 76期号:3页码:682-691 |
| 关键词 | Victoria Harbor pollution sulfate-reducing bacteria dsrAB T-RFLP clone library |
| 英文摘要 | Sulfate-reducing bacteria (SRB) are important regulators of a variety of processes in coastal marine sediments regarding organic matter turnover, biodegradation of pollutants, and sulfur and carbon cycles. Yet their community compositions have not been investigated in polluted harbor sediments. This study described the diversity and spatial variation of SRB communities in surface sediments in Victoria Harbor, Hong Kong. The spatial variation of SRB communities was described by terminal restriction fragment length polymorphism (T-RFLP). The results showed that the most diversified terminal restriction fragments were found at polluted sites. In addition, cluster analysis indicated that although the SRB communities were different at the two polluted sites, they were still more similar to each other than to the two more distant reference sites. Based on a dsrAB clone library constructed at a polluted site, diversified SRB were found, represented by 30 Operational Taxonomic Units (OTUs). Upon comparisons among the SRB sequences detected from this study and those in the GenBank, five clades of SRB were found. Three clades belonged to the known families Desulfobacteraceae, Desulfobulbaceae, and Syntrophobacteriaceae. The majority of sequenced clones, which distantly related to sequences in the GenBank, constituted the remaining two unclassified groups, suggesting unique SRB members related to the polluted harbor environment. Statistical analyses indicated that estimated SRB richness correlated with environment factors such as sulfur content, acid volatile sulfate, and redox potential. (C) 2007 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253085200021 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32633]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China 2.Hong Kong Univ Sci & Technol, Atmospher Marine & Coastal Environm Program, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Beijing, Peoples R China 4.Hong Kong Polytech Univ, Environm Engn Unit, Dept Civil & Struct Engn, Kowloon, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wen,Song, Lin-Sheng,Ki, Jang-Seu,et al. Microbial diversity in polluted harbor sediments II: Sulfate-reducing bacterial community assessment using terminal restriction fragment length polymorphism and clone library of dsrAB gene[J]. ESTUARINE COASTAL AND SHELF SCIENCE,2008,76(3):682-691. |
| APA | Zhang, Wen,Song, Lin-Sheng,Ki, Jang-Seu,Lau, Chun-Kwan,Li, Xiang-Dong,&Qian, Pei-Yuan.(2008).Microbial diversity in polluted harbor sediments II: Sulfate-reducing bacterial community assessment using terminal restriction fragment length polymorphism and clone library of dsrAB gene.ESTUARINE COASTAL AND SHELF SCIENCE,76(3),682-691. |
| MLA | Zhang, Wen,et al."Microbial diversity in polluted harbor sediments II: Sulfate-reducing bacterial community assessment using terminal restriction fragment length polymorphism and clone library of dsrAB gene".ESTUARINE COASTAL AND SHELF SCIENCE 76.3(2008):682-691. |
入库方式： OAI收割
来源：海洋研究所
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