Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''
文献类型：期刊论文
| 作者 | Wang, Jinfeng1,2 ; Jiang, Peng1; Zhou, Wei3; Cui, Yulin1,2; Qin, Song1
|
| 刊名 | BOTANICA MARINA
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 53期号:1页码:63-68 |
| 关键词 | DNA extraction ITS Porphyra rbcL shell-boring "Conchocelis'' |
| 英文摘要 | The genome DNA required for molecular studies of Porphyra has generally been extracted from either blades or free-living "Conchocelis''. However, the main disadvantages are complicated procedures, high cost, and DNA contamination. In this study, we developed a strategy for pure DNA extraction from shell-boring "Conchocelis'' and a single-step technique to prepare PCR templates. The results show that contamination of free-living "Conchocelis'' with microalgae was eliminated after the filaments had bored into shells. The electrophoretic banding patterns and intensities were similar between materials obtained from free-living "Conchocelis'' and shell-boring "Conchocelis'', suggesting that total DNA extraction from the latter was efficient. Molecular evidence demonstrated additional bands in PCR products of internal transcribed spacer (ITS) amplifications prepared from cultures of free-living "Conchocelis'' contaminated with microalgae. The additional bands appeared in all three Porphyra yezoensis replicates and one out of three Porphyra haitanensis replicates, but were not detectable in preparations from shell-boring "Conchocelis''. The DNA extracted from shell-boring "Conchocelis'' reduced the risk of microalgal contamination and the single-step technique reduced the time required for the preparation of DNA for molecular studies of Porphyra. The single-step method should be useful for PCR amplification of small amounts of materials, large sample sizes, or samples contaminated with microalgae. The technique may also be suitable for other genera of Bangiaceae, providing that shell-boring "Conchocelis'' stages are present in their life cycles. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274192000006 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32661]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Marine Fisheries Inst Jiangsu, Nantong 226007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jinfeng,Jiang, Peng,Zhou, Wei,et al. Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''[J]. BOTANICA MARINA,2010,53(1):63-68. |
| APA | Wang, Jinfeng,Jiang, Peng,Zhou, Wei,Cui, Yulin,&Qin, Song.(2010).Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''.BOTANICA MARINA,53(1),63-68. |
| MLA | Wang, Jinfeng,et al."Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''".BOTANICA MARINA 53.1(2010):63-68. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。