Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)
文献类型：期刊论文
| 作者 | Fu, Wandong1,2; Li Shuai3; Yao, Jianting1; Yu, Shenhui1; Liu, Fuli1; Duan, Delin1 |
| 刊名 | PLANT MOLECULAR BIOLOGY REPORTER
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 28期号:3页码:430-437 |
| 关键词 | Enteromorpha prolifera Heat shock protein 70 Molecular cloning |
| 英文摘要 | Heat shock protein 70 (Hsp70) is one of the important members of heat shock protein (Hsp) families and plays essential roles in folding nascent protein, translocation, refolding denatured protein, protein degradation, adverse stress resistance, and so on. In this study, homologous cloning coupled with the rapid amplification of cDNA ends was used to clone full-length cytosolic heat shock protein 70 of Enteromorpha prolifera (designed as EPHsp70). Bioinformatics was used to analyze structural feature, homologous relationship, and phylogenetic position of EPHsp70. The full length of EPHsp70 cDNA was 2,265 bp, with a 5' untranslated region of 65 bp, a 3' untranslated region of 217 bp, and an open-reading frame of 1,983 bp encoding a polypeptide of 660 amino acids with an estimated molecular weight of 71.39 kDa and an estimated isoelectric point of 5.03. EPHsp70 had five degenerate repeats of tetrapeptide GGMP and three typical Hsp70 signature motifs. The C-terminus amino acid sequence of cytosolic EPHsp70 was EEVD, and the conservation of EPHsp70 of N-terminus was higher than that of C-terminus. The homology between EPHsp70 and the cytosolic Hsp70s of other algae and land plants was more than 70%. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276440500009 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32687]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Zhejiang Marine Dev Res Inst, Zhoushan 316100, Peoples R China 3.Qingdao Univ, Coll Chem Chem Engn & Environm Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Wandong,Li Shuai,Yao, Jianting,et al. Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)[J]. PLANT MOLECULAR BIOLOGY REPORTER,2010,28(3):430-437. |
| APA | Fu, Wandong,Li Shuai,Yao, Jianting,Yu, Shenhui,Liu, Fuli,&Duan, Delin.(2010).Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta).PLANT MOLECULAR BIOLOGY REPORTER,28(3),430-437. |
| MLA | Fu, Wandong,et al."Molecular Cloning and Analysis of a Cytosolic Hsp70 Gene from Enteromorpha prolifera (Ulvophyceae, Chlorophyta)".PLANT MOLECULAR BIOLOGY REPORTER 28.3(2010):430-437. |
入库方式： OAI收割
来源：海洋研究所
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